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Lab Dept:
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Chemistry
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Test Name:
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BIOTINIDASE
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General Information
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Lab Order Codes:
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BIT
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Synonyms:
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Biotin-amide amidohydrolase; Biotinidase deficiency; Biotinidase, serum
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CPT Codes:
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82261 – Biotinidase, each specimen
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Test Includes:
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Biotinidase level reported in U/L.
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Logistics
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Test Indications:
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Useful for screening for biotinidase deficiency (preferred method) and for follow-up testing for certain organic acidurias.
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Lab Testing Sections:
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Chemistry - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# BIOTS/88205)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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4 - 8 days, test set up Monday and Thursday
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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3 mL (Minimum: 0.6 mL) blood
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Processed Volume:
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1 mL (Minimum: 0.2 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquot immediately into a plastic screw-capped vial. Store and ship at frozen temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Mislabeled or unlabeled specimens; gross hemolysis
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Interpretive
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Reference Range:
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3.5 - 13.8 U/L
Partial deficiencies and carriers may occur at the low end of the reference range. Suggest maintenance on a biotin supplementation and retesting in 1 month.
Values <3.5 U/L are occasionally seen in specimens from unaffected patients.
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Critical Values:
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N/A
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Limitations:
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A diet high in biotin may result in normal clinical presentation even when the biotinidase level is low.
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Methodology:
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Colorimetric
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References:
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Mayo Medical Laboratories Web Page (May 2012)
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Updates:
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7/13/2006: Specimen type change, previously listed as frozen serum.
6/17/2012: Specimen type change, previously listed as whole blood.
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