Clinics and Departments

Laboratory Services

Lab Dept:

Chemistry

Test Name:

BIOTINIDASE

General Information

Lab Order Codes:

BIT

Synonyms:

Biotin-amide amidohydrolase; Biotinidase deficiency; Biotinidase, serum

CPT Codes:

82261 – Biotinidase, each specimen

Test Includes:

Biotinidase level reported in U/L.

Logistics

Test Indications:

Useful for screening for biotinidase deficiency (preferred method) and for follow-up testing for certain organic acidurias.

Lab Testing Sections:

Chemistry - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: BIOTS/88205)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

4 - 8 days, test set up Monday and Thursday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

3 mL (Minimum: 0.6 mL) blood

Processed Volume:

1 mL (Minimum: 0.2 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot immediately into a plastic screw-capped vial. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimens; gross hemolysis

Interpretive

Reference Range:

3.5 - 13.8 U/L

Partial deficiencies and carriers may occur at the low end of the reference range. Suggest maintenance on a biotin supplementation and retesting in 1 month.

Values <3.5 U/L are occasionally seen in specimens from unaffected patients.

Critical Values:

N/A

Limitations:

A diet high in biotin may result in normal clinical presentation even when the biotinidase level is low.

Methodology:

Colorimetric

References:

Mayo Medical Laboratories Web Page (May 2012)

Updates:

7/13/2006: Specimen type change, previously listed as frozen serum.
6/17/2012: Specimen type change, previously listed as whole blood.



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