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Lab Dept:
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Chemistry
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Test Name:
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GROWTH HORMONE, SINGLE, SERUM
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General Information
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Lab Order Codes:
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HGH
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Synonyms:
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HGH; Somatotropin
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CPT Codes:
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83003 - Growth hormone, human (HGH)
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Test Includes:
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Single growth hormone level reported in ng/mL.
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Logistics
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Test Indications:
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Excess Growth Hormone secretion is associated with acromegaly in adults or pituitary gigantism in children, while a deficiency may cause short stature in children.
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Lab Testing Sections:
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Chemistry (St. Paul Campus)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 - 3 days, test performed Monday - Friday
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Special Instructions:
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Follow physician’s protocol for administering provocative stimuli and drawing specimens. See Patient Preparation.
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top (plain, no gel) tube
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Draw Volume:
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1.5 mL (Minimum: 0.6 mL) blood
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Processed Volume:
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0.5 mL (Minimum: 0.2 mL) serum
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Collection:
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Routine venipuncture or line draw
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Special Processing:
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Lab Staff: Centrifuge specimen within 1 hour of draw, remove serum aliquot into a screw-capped round bottom plastic vial. Freeze immediately. Ship to St. Paul frozen.
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Patient Preparation:
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See Special Instructions. In addition, patient must be fasting and at
complete rest 30 minutes before blood collection.
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Sample Rejection:
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Mislabeled or unlabeled specimens; improper storage; gross hemolysis; improper storage
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Interpretive
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Reference Range:
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Age
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Result Range
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0 - 1 day
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5 - 53 ng/mL
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1 day - 1 week
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5 - 27 ng/mL
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2 weeks - 12 months
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2 - 10 ng/mL
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>12 months:
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0 – 6 ng/mL
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Response Testing (Children and Adults):
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Following provocative stimuli, a normal response is a rise to 10 ng/mL or greater. Intermediate values between 7 ng/mL and 10 ng/mL may indicate a partial deficiency and require further evaluation.
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Critical Values:
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N/A
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Limitations:
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Patients on hGH therapy may develop human antibodies to hGH that will interfere with the test and cause falsely low levels.
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Methodology:
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Chemiluminescent competitive immunoassay
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References:
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DPC End User Procedure Manual Disk #0408111 (2005)
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Siemens Immulite Recombinant 98/574 hGH product insert (09/08/2010) PIL2KGRH-7
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National Committee for Clinical Laboratory Standards (NCCLS) (2002) Clinical Laboratory Technical Procedure Manuals; Approved Guideline-Fourth Edition GP2-A4
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Expected Values and S.I. Unit Conversion Tables, 2nd Edition, Endocrine Sciences, 4301 Lost Hills Rd, Calabasas Hills, CA 91301
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Biorad Immunoassay Plus Control Product Insert
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Burtis, CA, Ashwood, ER, Bruns, DE, ed (2006) Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 4th Edition, Philadelphia: W.B. Saunders
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Jacobs and DeMott Laboratory Test handbook (2001) 5th Edition, Lexi-comp Inc
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