Lab Dept:

Chemistry

Test Name:

5’ NUCLEOTIDASE, SERUM

General Information

Lab Order Codes:

5NT

Synonyms:

5 Nucleotidase; Nucleotidase

CPT Codes:

83915 – Nucleotidase 5

Test Includes:

5’ Nucleotidase level measured in U/L.

Logistics

Test Indications:

Determining cholestatic liver disease, particularly when GGT and ALP could be falsely elevated due to drug induction.

Lab Testing Sections:

Chemistry - Sendouts

Referred to:

Mayo Medical Laboratories (Test# 8692)

Phone Numbers:

Minneapolis:

Saint Paul:

612-813-6280

651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

2 - 9 days, test set up on Monday and Thursday

Special Instructions:

Fasting (4 hour fast) specimen preferred.

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

3.0 mL (Minimum: 1.5 mL) blood

Processed Volume:

1.0 mL (Minimum: 0.2 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Specimens other than serum, moderate to gross hemolysis, lipemia, mislabeled or unlabeled specimens

Interpretive

Reference Range:

2.0 – 8.0 U/L

Critical Values:

N/A

Limitations:

May be elevated in hyperammonemia due to interference in the method.

Methodology:

Enzyme Kinetic

Contraindications:

N/A

References:

Mayo Medical Laboratories Web Page (March 2009)

Updates:

3/24/2009: Reference range updated, previously listed as 4.0 – 11.5 U/L