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Lab Dept:
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Chemistry
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Test Name:
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ALKALINE PHOSPHATASE ISOENZYMES
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General Information
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Lab Order Codes:
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ALFR
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Synonyms:
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ALK Isoenzymes, serum; Alkaline Phosphatase Fractionation; ALP Isoenzymes; Regan Isoenzymes
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CPT Codes:
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84080 – Alkaline phosphatase; isoenzymes
84075 – Alkaline phosphatase; total
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Test Includes:
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Alkaline phosphatase, total; Alkaline phosphatase, isoenzymes (bone, liver and other fraction) reported in U/L and IU/L.
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Logistics
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Test Indications:
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Useful for determining the tissue source of elevated total alkaline phosphatase activity in serum. Differentiating between a liver and bone source of ALP. Diagnosis and treatment of liver, bone, intestinal, and parathyroid diseases.
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Lab Testing Sections:
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Chemistry - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 89503)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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2 – 4 days, Monday - Friday
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Special Instructions:
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None
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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3 mL blood
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Processed Volume:
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1 mL (Minimum: 0.5 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquots and place into TWO screw-capped round bottom plastic vials (Recommended: 0.5 mL into each vial - Minimum: 0.25 mL into each vial). Store and ship at frozen temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Hemolysis; warm specimens; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Total Alkaline Phosphatase (U/L)
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Age:
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Male:
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Female:
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<4 years
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Not established
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Not established
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4 years
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149 - 369 U/L
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169 - 372 U/L
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5 years
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179 - 416 U/L
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162 - 355 U/L
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6 years
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179 - 417 U/L
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169 - 370 U/L
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7 years
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172 - 405 U/L
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183 - 402 U/L
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8 years
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169 - 401 U/L
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199 - 440 U/L
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9 years
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175 - 411 U/L
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212 - 468 U/L
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10 years
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191 - 435 U/L
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215 - 476 U/L
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11 years
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185 - 507 U/L
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178 - 526 U/L
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12 years
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185 - 562 U/L
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133 - 485 U/L
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13 years
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182 - 587 U/L
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120 - 449 U/L
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14 years
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166 - 571 U/L
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153 - 362 U/L
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15 years
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138 - 511 U/L
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75 - 274 U/L
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16 years
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102 - 417 U/L
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61 - 264 U/L
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17 years
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69 - 311 U/L
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18 years
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52 - 222 U/L
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≥19 years
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45 - 115 U/L
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17 - 23 years
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52 - 144 U/L
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24 - 45 years
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37 - 98 U/L
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46 - 50 years
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39 - 100 U/L
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51 - 55 years
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41 - 108 U/L
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56 - 60 years
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46 - 118 U/L
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61 - 65 years
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50 - 130 U/L
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≥66 years
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55 - 142 U/L
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Isoenzymes:
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Age
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Liver Fraction 1 (%)
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Liver Fraction 1 (IU/L)
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0 - 6 years
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5.1 - 49%
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7.0 - 112.7 IU/L
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7 - 9 years
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3.0 - 45.0%
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7.4 - 109.1 IU/L
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10 - 13 years
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2.9 - 46.3%
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7.8 - 87.6 IU/L
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14 - 15 years
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7.8 - 48.9%
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10.3 - 75.6 IU/L
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16 - 18 years
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14.9 - 50.5%
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13.7 - 78.5 IU/L
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≥19 years
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27.8 - 76.3%
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16.2 - 70.2 IU/L
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Age
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Live Fraction 2 (%)
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Liver Fraction 2 (IU/L)
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0 - 6 years
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2.9 - 13.7%
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3.0 - 41.5 IU/L
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7 - 9 years
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3.7 - 12.5%
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4.0 - 35.6 IU/L
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10 - 13 years
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2.9 - 22.3%
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3.3 - 37.8 IU/L
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14 - 15 years
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2.2 - 19.8%
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2.2 - 32.1 IU/L
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16 - 18 years
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1.9 - 12.5%
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1.4 - 19.7 IU/L
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≥19 years
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0.0 - 8.0%
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0.0 - 5.8 IU/L
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Age
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Bone Fraction (%)
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Bone Fraction (IU/L)
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0 - 6 years
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41.5 - 82.7%
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43.5 - 208.1 IU/L
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7 - 9 years
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39.9 - 85.8%
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41.0 - 258.3 IU/L
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10 - 13 years
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31.8 - 91.1%
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39.4 - 346.1 IU/L
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14 - 15 years
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30.6 - 85.4%
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36.4 - 320.5 IU/L
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16 - 18 years
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38.9 - 72.6%
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32.7 - 214.6 IU/L
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≥19 years
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19.1 - 67.7%
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12.1 - 42.7 IU/L
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Age
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Intestine Fraction (%)
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Intestine Fraction (IU/L)
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0 - 6 years
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0.0 - 18.4 %
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0.0 - 37.7 IU/L
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7 - 9 years
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0.0 - 18.3 %
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0.0 - 45.6 IU/L
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10 - 13 years
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0.0 - 11.8%
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0.0 - 40.0 IU/L
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14 - 15 years
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0.0 - 8.2%
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0.0 - 26.4 IU/L
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16 - 18 years
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0.0 - 8.7%
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0.0 - 12.7 IU/L
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≥19 years
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0.0 - 20.6%
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0.0 - 11.0 IU/L
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Placental Fraction (all ages)
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Not present
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Critical Values:
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N/A
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Limitations:
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None
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Methodology:
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Total Alkaline Phosphatase: Photometric, p-Nitrophenol Phosphatase
Isoenzymes: Electrophoreses
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Contraindications:
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N/A
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References:
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Mayo Medical Laboratories Web Page (April 2011)
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Updates:
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9/26/2007: Test now forwarded to ARUP laboratories. Previously performed at Mayo Medical Laboratories. Note changes in isoenzymes and reference ranges.
12/7/2008: Test done internally at Mayo. New reference ranges.
4/19/2011: Added comment, no ranges established for <4 year on ALP Total.
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