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Lab Dept:
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Chemistry
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Test Name:
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ANTIDIURETIC HORMONE (ADH)
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General Information
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Lab Order Codes:
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ADH
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Synonyms:
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Arginine Vasopressin; AVP
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CPT Codes:
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84588 – Vasopressin (antidiuretic hormone, ADH)
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Test Includes:
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ADH concentration reported in pg/mL.
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Logistics
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Test Indications:
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Diagnosis and characterization of diabetes insipidus (DI). Diagnosis of psychogenic water intoxication and ectopic Arginine Vasopressin production, particularly due to bronchogenic carcinoma. As an adjunct in the diagnosis of inappropriate ADH syndrome, which results in dilutional hyponatremia.
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Lab Testing Sections:
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Chemistry - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 80344)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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3 - 11 days, test set up Wednesday
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Special Instructions:
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See Patient Preparation
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Specimen
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Specimen Type:
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Blood
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Container:
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Lavender top (EDTA) tube
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Draw Volume:
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5 mL (Minimum: 3.5 mL) blood
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Processed Volume:
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2mL (Minimum: 1.15 mL) plasma
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen at approximately 1000 G for 10 minutes in a refrigerated centrifuge, remove plasma aliquot avoiding the platelet/buffy coat. Place plasma aliquot in a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.
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Patient Preparation:
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Patient should fast and thirst for 6 hours (preferred). No liquids, including water, are allowed.
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Sample Rejection:
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Specimens containing radioactive isotopes; specimens warm for more than 4 hours; specimens other than plasma, mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Adults: <1.7 pg/mL
Reference values were determined on platelet-poor EDTA plasma from individuals fasting no longer than overnight.
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Critical Values:
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N/A
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Limitations:
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Reference values were determined on platelet-poor EDTA plasma from individuals fasting no longer than overnight. A significant amount of circulating AVP is associated with platelets. Therefore, various conditions affecting platelets may also affect AVP levels. Platelet-rich specimens have been shown to have AVP levels on the order of 10 times the value of platelet-poor specimens.
AVP levels obtained in the process of a water deprivation test may be difficult to interpret because of the many nonstandardized variables in this test. Expert consultation is recommended in these circumstances.
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Methodology:
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This method uses a cartridge extraction of acidified plasma to prepare the specimen for an in-house radioimmunoassay (RIA). After acidification, the specimen is centrifuged, applied to a phenyl cartridge, washed, and eluted. The extract is dried under nitrogen, reconstituted with assay buffer and measured for AVP by RIA.
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References:
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Mayo Medical Laboratories (September 2010)
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Updates:
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2/26/2004: Test moved from Specialty Laboratories to Mayo Medical Labs.
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