Clinics and Departments

Laboratory Services

Lab Dep:

Chemistry

Test Name:

VITAMIN D, 25-HYDROXY

General Information

Lab Order Codes:

VDT

Synonyms:

Vitamin D Assay; 25-Hydroxycholecalciferol; 25-OH-Vitamin D; 25-Hydroxy D2, 25-Hydroxy D3; Calcidiol

CPT Codes:

82306 – Calcifediol (25-OH Vitamin D-3)

Test Includes:

Total 25-hydroxyvitamin measured in ng/mL.

Logistics

Test Indications:

The test is used to assess patient’s vitamin D status, evaluate hypo and hypercalcemia and to investigate the causes of osteomalacia and rickets. Also useful in monitoring vitamin D replacement therapy.

Lab Testing Sections:

Chemistry (Performed on the St. Paul Campus)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 3 days, performed Monday - Friday

Special Instructions:

See Patient Preparation

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1.5 mL blood

Processed Volume:

0.5 mL (Minimum: 0.25 mL) serum

Collection:

Routine venipuncture, capillary specimens are not recommended

Special Processing:

Lab Staff: Centrifuge specimen when clotted, remove serum aliquot into a screw-capped plastic vial. Store at refrigerated temperatures for up to 5 days.

Patient Preparation:

Fasting samples are recommended, but not required

Sample Rejection:

Specimens other than serum; unlabeled or mislabeled specimens

Interpretive

Reference Range:

All ages: 30 – 100 ng/mL

Critical Values:

N/A

Limitations:

● Gross hemolysis falsely elevates Vitamin D.
● The test cross-reacts with dihydroxylated metabolites of Vitamin D.

Methodology:

Direct competitive chemiluminescent immunoassay

References:

Liason 25 OH Vitamin D, Product Insert 1/2009, DiaSorin, Inc.



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