Clinics and Departments

Laboratory Services

Lab Dept:

Chemistry

Test Name:

PENTOBARBITAL

General Information

Lab Order Codes:

PENT

Synonyms:

Nembutal®, Sodium Pentobarbital

CPT Codes:

82491 – Chromatography, quantitative, column; single analyte not elsewhere specified, single stationary and mobile phase

Test Includes:

Pentobarb level reported in ug/mL.

Logistics

Test Indications:

Therapeutic sedative/hypnotic monitoring

Lab Testing Sections:

Chemistry - Sendouts

Referred to:

MedTox Laboratories (MedTox Test: 90)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1-3 days

Special Instructions:

Trough levels are most reproducible.

Specimen

Specimen Type:

Blood

Container:

Green top (Li Heparin) or Red top tube

Draw Volume:

6 mL (Minimum: 1 mL) blood

Processed Volume:

2 mL (Minimum 0.3 mL) serum/plasma

Note: Submission of the minimum volume results in an elevated reporting range and inability to repeat analysis.

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum/plasma aliquot into a screw-capped round bottom plastic vial. Store and ship at refrigerated temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimens

Interpretive

Reference Range:

1.0 – 5.0 ug/mL

Critical Values:

>50.0 ug/mL

Note: Pentobarbital concentrations as high as 50 ug/ml may be required to induce therapeutic coma.

Limitations:

<0.3 ug/mL reporting limit

Methodology:

Gas Chromatography/Nitrogen Phosphorus Detection (GC-NPD)

References:

MedTox Laboratories (May 2010)

Updates:

5/6/2010: CPT Update, previously reported as 82205 – Barbituates, not elsewhere specified



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