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Lab Dept:
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Chemistry
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Test Name:
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PYRUVATE KINASE, RBC
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General Information
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Lab Order Codes:
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PYKI
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Synonyms:
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Pyruvate Kinase, Erythrocytes
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CPT Codes:
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84220 – Pyruvate kinase
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Test Includes:
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Pyruvate kinase RBC level reported in U/g Hb.
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Logistics
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Test Indications:
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Useful for the workup of cases of nonspherocytic hemolytic anemia, for a family workup to determine inheritance pattern (pyruvate kinase deficiency is autosomal recessive), and for genetic counseling.
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Lab Testing Sections:
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Chemistry - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 8659)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 - 4 days, test set up Monday - Saturday
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Yellow top (ACD- Solution B) tube
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Draw Volume:
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4 mL (Minimum: 1 mL) blood
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Processed Volume:
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Same as Draw Volume – Do Not process
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Do Not centrifuge. Send whole blood refrigerated in original collection container. Do Not transfer blood to other containers. Store and ship at refrigerated temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Specimen cannot be frozen, mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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6.7 – 14.3 U/g Hb
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Critical Values:
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N/A
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Limitations:
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Because leukocytes also contain pyruvate kinase that is not diminished in hereditary erythrocytic pyruvate kinase deficiency, freeing the blood of white blood cells is always critical to this test.
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Methodology:
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Kinetic Spectrophotometry (KS)
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References:
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Mayo Medical Laboratories (August 2010)
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Updates:
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8/31/2010: Units and reference range update.
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