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Lab Dept:
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Chemistry
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Test Name:
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VITAMIN D, 1,25 DIHYDROXY
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General Information
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Lab Order Codes:
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D125
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Synonyms:
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1,25-Dihydroxycholecalciferol; Calcitriol
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CPT Codes:
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82652 – Dihydroxyvitamin D, 1, 25-
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Test Includes:
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Vitamin D, 1, 25-Dihydroxy level reported in pg/mL.
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Logistics
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Test Indications:
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As a second-order test in the assessment of vitamin D status, especially in patients with renal disease.
Investigation of some patients with clinical evidence of vitamin D deficiency (eg, vitamin D-dependent rickets due to hereditary deficiency of renal 1-alpha hydroxylase or end-organ resistance to 1,25-dihydroxy vitamin D)
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Lab Testing Sections:
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Chemistry - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 8822)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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2 - 4 days, test set up Monday – Friday.
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Special Instructions:
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See Patient Preparation
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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4.5 mL (Minimum: 2.1 mL) blood
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Processed Volume:
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1.5 mL (Minimum: 0.7 mL) serum
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Collection:
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Routine venipuncture. Forward to laboratory promptly.
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at refrigerated temperatures. Forward promptly.
Note: Specimens submitted less that the minimum of 1.2 mL will result in a phone call requesting additional specimen. If more specimen is not available, testing will be canceled.
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Patient Preparation:
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Patient should be fasting at least 4 hours, recommended but not required.
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Sample Rejection:
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Excessive hemolysis, mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Females
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0 – 15 years:
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24 – 86 pg/mL
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≥16 years:
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18 – 78 pg/mL
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Males
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0 – 15 years:
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24 – 86 pg/mL
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≥16 years:
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18 – 64 pg/mL
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Critical Values:
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N/A
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Limitations:
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N/A
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Methodology:
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Extraction/Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
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References:
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Mayo Medical Laboratories Web Page (April 2009)
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Updates:
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7/11/2008: Minimum volume serum increase from 0.65 to 1.2 mL.
11/20/2008: Specimen handling for short specimens.
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