Clinics and Departments

Laboratory Services

Lab Dept:

Chemistry

Test Name:

DIHYDROTESTOSTERONE

General Information

Lab Order Codes:

DHT

Synonyms:

DHT

CPT Codes:

82542 – Column chromatography/mass spectrometry, quantitative, single stationary and mobile phase

Test Includes:

Dihydrotestosterone level reported in ng/mL.

Logistics

Test Indications:

Serum DHT is an important steroid test for children, women and men because it is the immediate and active metabolite of testosterone. Conversion of testosterone to DHT occurs in target tissue by the action of 5a-reductase. Levels are normally low in children, but may be elevated in cases of steroid metabolic disease such as CAH (Congenital Adrenal Hyperplasia). Elevated levels in women may indicate ovarian disease. Low levels relative to testosterone may indicate partial or complete inactivity of 5a-reductase.

Lab Testing Sections:

Chemistry - Sendouts

Referred to:

Esoterix, Inc. (ESL Test: 500144)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1-3 days, test is set up Tuesday through Saturday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top (plain, no gel) tube

Draw Volume:

3 mL (Minimum: 1.5 mL) blood

Processed Volume:

1 mL (Minimum: 0.5 mL) serum

Note: Submission of the minimum amount will not allow for repeat analysis.

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen within one hour of draw. Remove serum aliquot into a screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimens

Interpretive

Reference Range:

Premature Infants:

Males:

10 – 53 ng/dL

Females:

2 – 13 ng/dL

Full Term Infants:

Males:

30 - 85 ng/dL

Females:

4 - 22 ng/dL

Infants (2 weeks – 6 months):

Males: DHT decreases rapidly the first week then increases to 12 – 85 ng/dL between 30 – 60 days. Levels then decrease gradually to prepubertal values by 7 months.

Females: Levels decrease during the first month to <3 ng/dL and remain there until puberty.

Prepubertal Children:

<3 ng/dL

Puberty:

 

Male

 

Female

 

Tanner Stage

AGE (years)

DHT ng/dL

Age (year)

DHT ng/dL

1

<9.8

<3

<9.2

<3

2

9.8 – 14.5

3 – 17

9.2 – 13.7

5 – 12

3

10.7 – 15.4

8 – 33

10.0 – 14.4

7 – 19

4

11.8 – 16.2

22 – 52

10.7 – 15.6

4 – 13

5

12.8 – 17.3

24 – 65

11.8 – 18.6

3 – 18

Adults:

Males:

30 – 85 ng/dL

Females:

4 – 22 ng/dL

Critical Values:

N/A

Limitations:

LLOQ (sensitivity) 1ng/dL

Methodology:

HPLC Tandem Mass Spectrometry

References:

Esoterix, Inc. “Expected Value & S.I. Unit Conversion Table” Fifth Edition, 2003

Esoterix, Inc. Web Page July 2012

Updates:

8/3/2009: Note updated reference ranges.
3/10/2014: CPT update, previously listed as 82651.



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