Clinics and Departments

Laboratory Services

Lab Dept:

Chemistry

Test Name:

TUMOR NECROSIS FACTOR, ALPHA, HIGHLY SENSITIVE

General Information

Lab Order Codes:

TNF

Synonyms:

Tumor Necrosis Factor Alpha, Cytokines

CPT Codes:

83520 - Immunoassay, analyte, quantitative; not otherwise specified

Test Includes:

Tumor necrosis factor, concentration reported in pg/mL.

Logistics

Test Indications:

Tumor necrosis factor (TNF-a, cachectin) was described based on the observation of hemorrhagic necrosis of tumors in animals and humans, as well as in patients with caxhexia. It is produced b mononuclear phagocytes, lymphocytes, natural killer cells, and some human tumor cells. Its effects have been studied in many cell types, including polymorphonuclear leukocytes, B- and T-lymphocytes, vascular endothelial cells, fibroblasts, hematopoietic tissues, adipocytes, hepatocytes, and bone and cartilage cells.

Lab Testing Sections:

Chemistry - Sendouts

Referred to:

Mayo Medical Laboratories forward to Quest Diagnostics Nichols Institute (MML test: FFTUM, Quest test: 34485)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 8 days, test performed each Tuesday AM

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top (plain, no gel) tube

Draw Volume:

3 mL (Minimum: 1.5.mL) blood

Processed Volume:

1 mL (Minimum: 0.5 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Allow specimen to clot for 10 - 15 minutes at room temperature. Specimens must be processed within 30 minutes of collection. Centrifuge and aliquot 1.0 mL (Minimum: 0.5 mL) serum into screw-capped round bottom plastic vials. Store in freezer. Ship frozen. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Thawed specimens; mislabeled or unlabeled specimens

Interpretive

Reference Range:

1.2 - 15.3 pg/mL

TNF- Alpha is not to be used as a diagnostic procedure without confirmation of the diagnosis by another established product or procedure. The reference range is intended to be used for blood samples only. Reference ranges for body fluids other than blood have not been established.

This test is performed using a kit that has not been approved or cleared by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics Nichols Institute. This test should not be used for diagnosis without confirmation by other medically established means.

Critical Values:

N/A

Limitations:

N/A

Methodology:

Enzyme Linked Immunosorbent Immunoassay

References:

Mayo Medical Laboratories Web Page (September 2013)

Quest Diagnostics Nichols Institute Web Page (September 2013)

Updates:

3/11/2004: Testing moved from Fairview Diagnostic Laboratories to Mayo Medical Laboratories forward to ARUP.
6/27/2007: Test moved to Forward to Quest from Mayo. Note new reference range and method.



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services