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Lab Dept:
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Chemistry
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Test Name:
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TUMOR NECROSIS FACTOR, ALPHA, HIGHLY SENSITIVE
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General Information
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Lab Order Codes:
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TNF
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Synonyms:
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Tumor Necrosis Factor Alpha, Cytokines
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CPT Codes:
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83520 - Immunoassay, analyte, quantitative; not otherwise specified
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Test Includes:
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Tumor necrosis factor, concentration reported in pg/mL.
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Logistics
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Test Indications:
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Tumor necrosis factor (TNF-a, cachectin) was described based on the observation of hemorrhagic necrosis of tumors in animals and humans, as well as in patients with caxhexia. It is produced b mononuclear phagocytes, lymphocytes, natural killer cells, and some human tumor cells. Its effects have been studied in many cell types, including polymorphonuclear leukocytes, B- and T-lymphocytes, vascular endothelial cells, fibroblasts, hematopoietic tissues, adipocytes, hepatocytes, and bone and cartilage cells.
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Lab Testing Sections:
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Chemistry - Sendouts
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Referred to:
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Mayo Medical Laboratories forward to Quest Diagnostics Nichols Institute (Test# 91729)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 8 days, test performed each Tuesday
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Red top (plain, no gel) tube
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Draw Volume:
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3.0 mL (Minimum: 1.5.mL) blood
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Processed Volume:
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1.0 mL (Minimum: 0.5 mL) serum/plasma
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Allow specimen to clot for 10 - 15 minutes at room temperature. Specimens must be processed within 30 minutes of collection. Centrifuge and aliquot 1.0 mL (Minimum: 0.5 mL) serum into screw-capped round bottom plastic vials. Store in freezer (-70°C preferred). Ship frozen. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Thawed specimens, mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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1.2 - 15.3 pg/mL
TNF- Alpha is not to be used as a diagnostic procedure without confirmation of the diagnosis by another established product or procedure.
The reference range is intended to be used for blood samples only. Reference ranges for body fluids other than blood have not be established.
This test is performed using a kit that has not been approved or cleared by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics Nichols Institute. This test should not be used for diagnosis without confirmation by other medically established means.
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Critical Values:
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N/A
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Limitations:
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N/A
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Methodology:
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Enzyme Linked Immunosorbent Immunoassay
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Contraindications:
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N/A
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References:
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Mayo Medical Laboratories Web Page (June 2007)
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Quest Diagnostics Nichols Institute Web Page (June 2007)
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Updates:
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3/11/2004: Testing moved from Fairview Diagnostic Laboratories to Mayo Medical Laboratories forward to ARUP.
6/27/2007: Test moved to Forward to Quest from Mayo. Note new reference range and method.
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