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Lab Dept:
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Chemistry
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Test Name:
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PROTEIN, TOTAL
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General Information
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Lab Order Codes:
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TP
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Synonyms:
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Serum Protein
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CPT Codes:
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84155 – Protein; total, except refractometry
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Test Includes:
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Total Protein concentration reported in g/dL.
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Logistics
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Test Indications:
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Useful for evaluating nutritional status, kidney disease, liver disease and many other conditions. If the total protein is abnormal, further tests must be performed to identify which protein fraction is abnormal to aid in identifying a specific diagnosis.
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Lab Testing Sections:
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Chemistry
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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2 hours
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Green top (Li Heparin) preferred
Alternate tube: Red top (plain, no gel) tube
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Draw Volume:
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0.6 mL blood
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Processed Volume:
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0.2 mL serum/plasma
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Collection:
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Routine venipuncture. Mix tubes containing anticoagulant by gentle inversion.
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum/plasma aliquot into a plastic sample cup. Analyze sample immediately or store at refrigerated temperatures.
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Patient Preparation:
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None
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Sample Rejection:
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Mislabeled or unlabeled specimen; gross hemolysis; grossly icteric
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Interpretive
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Reference Range:
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Age:
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Range (g/dL)
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0 - 4 months:
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3.6 - 7.4
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4 months - 1 year:
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4.3 - 7.3
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1 - 2 years:
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5.6 - 7.2
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>2 years:
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6.0 - 8.0
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Critical Values:
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N/A
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Limitations:
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Excess patient hemolysis increases the total protein value, while excess bilirubin will decrease it.
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Methodology:
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Biuret Reaction
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References:
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Jacobs and DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc., Hudson, OH, 5th ed
Total Protein Flex reagent cartridge insert sheet (04-05-2010) PN 717073.001-US
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Updates:
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12/22/2011: Turnaround time updated, previously listed as 4 hours.
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