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Lab Dept:
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Chemistry
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Test Name:
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GENTAMICIN LEVEL, SERUM
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General Information
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Lab Order Codes:
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GENT
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Synonyms:
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Garamycin®
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CPT Codes:
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80170 – Gentamicin
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Test Includes:
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Gentamicin concentration in ug/mL
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Logistics
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Test Indications:
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Useful in monitoring therapeutic dosage and is affective against gram-negative bacteria.
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Lab Testing Sections:
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Chemistry
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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4 hours
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Special Instructions:
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A peak level should be drawn 30-60 minutes after infusion. A trough level should be drawn just before the next dose
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Specimen
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Specimen Type:
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Blood
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Container:
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Green top (Li Heparin) tube preferred
Alternate tube: Red top (plain, no gel) tube
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Draw Volume:
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0.6 mL blood
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Processed Volume:
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0.2 mL serum/plasma
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Collection:
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Routine venipuncture. Mix tubes containing anticoagulant by gentle inversion.
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum/plasma aliquot into plastic sample cup. Store at refrigerated temperatures.
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Patient Preparation:
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None
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Sample Rejection:
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Mislabeled or unlabeled specimen
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Interpretive
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Reference Range:
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Peak Levels
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Therapeutic concentration:
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4 – 8 ug/mL
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Trough Levels
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Therapeutic concentration:
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<2 ug/mL generally indicates elimination of the drug
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Critical Values:
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Toxic concentration peak: >10 ug/mL
Toxic concentration trough: >2 ug/mL for >10 days
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Limitations:
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Aminoglycosides similar in structure may cross-react with this assay.
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Methodology:
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Particle Enhanced Turbidimetric Inhibition Immunoassay (Petinia)
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References:
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GENT Flex™ reagent cartridge insert sheet, Siemens Healthcare Diagnostics (2008-03-03) Rev. F 717012.001 - US
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Clinical Significance, Dade Behring Inc., Glasgow Business Community, Mailbox 531, P.O. Box 6101, Newark, Delaware 19714
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Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition, 2001
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Biorad Liquichek Unassayed Chemistry Control Product Insert, Bio-Rad Laboratories, Irvine, CA 92618
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