|
|
|
Lab Dept:
|
Chemistry
|
Test Name:
|
TOBRAMYCIN LEVEL, SERUM
|
General Information
|
Lab Order Codes:
|
TOBR
|
Synonyms:
|
Nebcin
|
CPT Codes:
|
80200 - Tobramycin
|
Test Includes:
|
Tobramycin concentration reported in ug/mL.
|
Logistics
|
Test Indications:
|
Useful for therapeutic drug monitoring (TDM). Tobramycin is an aminoglycoside, which is a polycationic agent that kills aerobic gram-negative bacteria.
|
Lab Testing Sections:
|
Chemistry
|
Phone Numbers:
Minneapolis:
Saint Paul:
|
|
612-813-6280
|
651-220-6550
|
Test Availability:
|
Daily, 24 hours
|
Turnaround Time:
|
4 hours
|
Special Instructions:
|
Peak levels should be drawn 30-60 minutes after infusion of the drug. Trough levels should be drawn just before next dose. Indicate whether specimen is a peak or trough level.
|
Specimen
|
Specimen Type:
|
Blood
|
Container:
|
Green top (Li Heparin) tube preferred
Alternate tube: Red top (plain, no gel) tube
|
Draw Volume:
|
0.6 mL blood
|
Processed Volume:
|
0.2 mL serum/plasma
|
Collection:
|
Routine venipuncture. Mix tubes containing anticoagulant by gentle inversion. Indicate peak or trough on specimen label.
|
Special Processing:
|
Lab Staff: Centrifuge specimen, remove serum/plasma aliquot into a plastic sample cup. Store at refrigerated temperatures.
|
Patient Preparation:
|
None
|
Sample Rejection:
|
Mislabeled or unlabeled specimen
|
Interpretive
|
Reference Range:
|
Peak
|
Therapeutic concentration:
|
4 - 8 ug/mL
|
Trough
|
Therapeutic concentration:
|
<2 ug/mL generally indicates elimination of the drug
|
Critical Values:
|
>10 ug/mL
|
Limitations:
|
Patients receiving Kanamycin and other structurally similar aminoglycosides should not rely on this method.
|
Methodology:
|
Particle Enhanced Turbidimetric Inhibition Immunoassay (PETINIA)
|
References:
|
TOBR Flex® reagent cartridge insert sheet Siemens Healthcare Diagnostics Inc (2010-01-22) PN 717014.001 – US, Rev G
|
www.childrensmn.org/Lexi-Comp Copyright © (1978-2006) Lexi-Comp Inc
|
Clinical Significance, Dade Behring Inc., Glasgow Business Community, Mailbox 531, P.O. Box 6101, Newark, Delaware 19714
|
Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition
|
Biorad Liquichek Unassayed Chemistry Control Product Insert, Bio-Rad Laboratories, Irvine, CA
|
