Clinics and Departments

Laboratory Services

Lab Dept:

Chemistry

Test Name:

LUTEINIZING HORMONE (LH)

General Information

Lab Order Codes:

LH

Synonyms:

LH; Lutropin

CPT Codes:

83002 – Gonadotropin; luteinizing hormone (LH)

Test Includes:

LH concentration measured in mIU/mL.

Logistics

Test Indications:

Luteinizing hormone (LH) is secreted by the anterior pituitary in response to gonadotropin-releasing hormone. LH secretion is regulated by a balance of positive and negative feedback mechanisms involving the hypothalamic-pituitary axis and the pituitary. FSH and LH play a critical role in maintaining the normal function of the male and female reproductive systems.

Lab Testing Sections:

Chemistry

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 day

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Preferred: Green top (Li Heparin) tube
Alternate: Red top (plain, no gel) tube

Draw Volume:

1.5 mL (Minimum: 0.6 mL) blood

Processed Volume:

0.5 mL (Minimum: 0.2 mL) plasma/serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove plasma/serum aliquot into a plastic sample cup. Store at 2-8 degrees Centigrade for up to 7 days.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimen

Interpretive

Reference Range:

Age:

Range: mIU/mL

Pediatrics (Prepubertal Children):

<5.0 mIU/mL

Adult Males:

1.5 – 9.0 mIU/mL

Menstruating Females:

Follicular phase:

2.0 – 9.0 mIU/mL

Midcycle peak:

17.5 – 72.9 mIU/mL

Luteal phase:

2.0 – 11.0 mIU/mL

Critical Values:

N/A

Limitations:

Heterophile antibodies may interfere with immunoassay tests.

Methodology:

Homeogeneous Sandwich Chemiluminescent Immunoassay

References:

Siemens Dimension Vista LH Instructions For Use. 8/4/2012

Jacobs and DeMott Laboratory Test Handbook (2001), Lexi-Comp Inc, Hudson, OH, 5th Edition

National Committee for Clinical Laboratory Standards (NCCLS) (2002) Clinical Laboratory Technical Procedure Manuals; Approved Guideline-Fourth Edition GP2-A4

Burtis CA, Ashwood ER, editors (2006) Tietz Textbook of Clinical Chemistry. 4th ed. Philadelphia: W.B. Saunders

Biorad Immunoassay Plus Control Product Insert PIL2KLH-19

Updates:

7/22/2014: Method change, previously listed as Competitive Chemiluminescent Immunoassay



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