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Lab Dept:
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Chemistry
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Test Name:
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IGE (IMMUNOGLOBULIN E)
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General Information
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Lab Order Codes:
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IGE
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Synonyms:
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Immunoglobulin E
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CPT Codes:
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82785 – Gammaglobulin; IgE
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Test Includes:
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IgE concentration reported in IU/mL.
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Logistics
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Test Indications:
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Immunoglobulin E is a trace protein in serum and its concentration is usually age dependant. IgE is so rapidly and firmly bound to mast cells that only trace amounts of it are normally present in the serum. IgE fights allergens in response to allergic reactions.
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Lab Testing Sections:
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Chemistry (all specimens analyzed on the Mpls campus)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 4 days, test performed Tuesday, Wednesday and Friday
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top (plain, no gel) tube
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Draw Volume:
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0.6 mL blood
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Processed Volume:
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0.2 mL serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen, remove serum aliquot into a plastic sample cup. Specimens can be stored at refrigerated temperatures. Centrifuge turbid or particulate samples prior to analysis.
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Patient Preparation:
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None
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Sample Rejection:
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Mislabeled or unlabeled specimen; specimens other than serum; turbid samples that do not clear after centrifugation
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Interpretive
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Reference Range:
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Age:
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Range (IU/mL):
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<1 year:
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0 – 15 IU/mL
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1 – 6 years:
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0 – 60 IU/mL
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7 – 10 years:
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0 – 90 IU/mL
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11 – 16 years:
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0 – 200 IU/mL
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>16 years:
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0 – 100 IU/mL
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Critical Values:
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N/A
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Limitations:
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N/A
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Methodology:
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Nephelometry, BN Prospec
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References:
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Winter WE, Hardt NS, Fuhrman S (2000) Immunoglobulin E: Importance in parasitic infections and hypersensitivity responses. Arch Pathol Lab Med; 124:1382-5.
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Biosafety in Microbiological and Biomedical Laboratories (1993) U. S. Department of Health and Human Services, Washington [HHS Publication No. (CDC) 93-8395]
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Dati, F & Ringel, KP (1982)Reference values for serum IgE in healthy non-atopic children and adults. Clin Chem; 28: 1556
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Ringel KP, Dati F, Buchholz E (1982) IgE-Normalwerte bei Kindern. Laboratoriumsblätter; 32:26–34
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National Committee for Clinical Laboratory Standards. Evaluation of Precision Performance of Clinical Chemistry Devices; Approved Guideline (1999) NCCLS document EP5-A (ISBN 1-56238-368-X). NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898, USA
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Tietz, NW (1986) Textbook of Clinical Chemistry, WB Saunders Co., Philadelphia, PA, pp 52-53 (techniques and procedures to minimize laboratory infections) and pp 478-497 (specimen collection and storage recommendations)
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N Latex IgE Mono Package Insert (May 2008) Siemens Healthcare Diagnostics, Newark, DE
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N Protein Standard SL Package Insert (May 2008) Siemens Healthcare Diagnostics, Newark, DE
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N Supplementary Reagent L Package Insert, Siemens Healthcare Diagnostics, Newark, DE, May 2008
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