Clinics and Departments

Laboratory Services

Lab Dept:

Chemistry

Test Name:

AST

General Information

Lab Order Codes:

AST

Synonyms:

Aspartate Aminotransferase; SGOT; Serum Glutamic Oxaloacetic Transaminase

CPT Codes:

84450 - Transferase; aspartate amino (AST) (SGOT)

Test Includes:

Aspartate aminotransferase concentration reported in U/L.

Logistics

Test Indications:

The aminotransferases constitute a group of enzymes that catalyze the interconversion of amino acids and 2-oxo-acids by transfer of amino acids. AST and ALT are the two enzymes of greatest clinical significance. Elevated AST levels are found in hepatic disease, myocardial damage, pulmonary emboli, muscle injuries, muscular dystrophy, hemolytic disease, acute pancreatitis, or gangrene.

Lab Testing Sections:

Chemistry

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

4 hours

Special Instructions:

N/A

Specimen

Specimen Type:

Whole blood

Container:

Preferred: Green top (Li Heparin) tube
Alternate: Red top (plain, no gel) tube

Draw Volume:

0.6 mL blood

Processed Volume:

0.2 mL plasma/serum

Collection:

Routine blood collection

Special Processing:

Lab Staff: Centrifuge specimen, transfer serum/plasma into plastic sample cup. Analyze sample immediately or store at refrigerated temperatures for up to 7 days.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimen; gross hemolysis

Interpretive

Reference Range:

Age:

Males & Females (U/L):

0 – 2 years:

10 – 74 U/L

2 – <8 years:

10 – 50 U/L

>8 years:

10 – 41 U/L

Critical Values:

N/A

Limitations:

Hemolysis of the sample falsely increases the AST concentration.

Methodology:

Enzymatic, 37C

References:

Vista AST reagent cartridge insert sheet, Siemens Healthcare Diagnostics Inc., 2013-07-16, Rev F, PN 781041.001 - US

AST Flex™ reagent cartridge insert sheet Siemens Healthcare Diagnostics Inc. 2012-07-13 Rev H, F 717041.002 - US

Dimension Vista Enzyme 2 Calibrator insert sheet, Siemens Healthcare Diagnostics Inc., PN 751321.001-US, Rev A, 01/2011

Enzyme Verifier insert sheet, Siemens Healthcare Diagnostics Inc., Newark, DE 19714, PN 792019.001-US, 01/2013, Rev I

Clinical Significance, Dade Behring Inc., Glasgow Business Community, Mailbox 531, P.O. Box 6101, Newark, Delaware 19714

Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition

Biorad Multiqual Assayed Chemistry Control Product Insert, Bio-Rad Laboratories, Irvine, CA 92618

Biorad Liquichek Unassayed Chemistry Control Product Insert, Bio-Rad Laboratories, Irvine, CA 92618

Pediatric Reference Ranges (2007), 6th Edition, Steven J Soldin, Carlo Brugnara, Edward C Wong, AACC Press, 7/1/2007

CLSI-based transference of the CALIPER database of pediatric reference intervals from Abbott to Beckman, Ortho, Roche and Siemens Clinical Chemistry Assays, Clinical Biochemistry 46 (2013) 1197-1219

Updates:

2/17/2005: Critical value previously listed >500 U/L.
8/17/2015: Method and ref range update.



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