Clinics and Departments

Laboratory Services

Lab Dept:

Chemistry

Test Name:

BILIRUBIN, DIRECT

General Information

Lab Order Codes:

DBIL

Synonyms:

Direct Bilirubin; Conjugated Bilirubin

CPT Codes:

82248 – Bilirubin; direct

Test Includes:

Direct bilirubin concentration reported in mg/dL.

Logistics

Test Indications:

Bilirubin is the orange-yellow pigment derived from senescent red blood cells. It is extracted and biotransformed in the liver. Bilirubin is screened and monitored for liver disorders, such as jaundice, or liver disease, such as cirrhosis.

Lab Testing Sections:

Chemistry

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 hour

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Preferred: Green top (Li Heparin) tube
Alternate: Red top (plain, no gel) tube

Draw Volume:

0.6 mL blood

Processed Volume:

0.2 mL plasma/serum

Collection:

Routine venipuncture or heelstick

Special Processing:

Lab Staff: Specimen must be separated from cells within 2 hours of collection. Centrifuge specimen, remove serum/plasma aliquot into plastic sample cup. Analyze sample immediately or store at refrigerated temperatures. Protect specimen from light when stored for more than 8 hours.

Patient Preparation:

Remove photo therapy prior to sample collection.

Sample Rejection:

Unlabeled or mislabeled specimen

Interpretive

Reference Range:

Age:

Range (mg/dL):

0 – 2 weeks:

0.0 – 1.2 mg/dL

>2 weeks:

0.0 – 0.3 mg/dL

Critical Values:

N/A

Limitations:

Hemolysis of the sample falsely decreases the direct bilirubin result. Exposure to light rapidly and significantly decreases bilirubin.

Methodology:

Modified Jendrassik-Grof

References:

DBI Flex® reagent cartridge insert sheet, Siemens Healthcare Diagnostics, PN 717125.001 Issue Date 04/07/2008 Rev. B

Clinical Significance, Dade Behring Inc., Glasgow Business Community, Mailbox 531, P.O. Box 6101, Newark, Delaware 19714

Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition

Biorad Liquichek Unassayed Chemistry Control Product Insert, Bio-Rad Laboratories, Irvine, CA 92618

Biorad Liquichek Pediatric Control Product Insert, Bio-Rad Laboratories, Irvine, CA 92618

Frequently Asked Questions (March 2008) Dade Behring, Global Products Support



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