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Lab Dept:
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Chemistry
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Test Name:
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BILIRUBIN, DIRECT
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General Information
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Lab Order Codes:
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DBIL
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Synonyms:
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Direct Bilirubin; Conjugated Bilirubin
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CPT Codes:
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82248 – Bilirubin; direct
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Test Includes:
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Direct bilirubin concentration reported in mg/dL.
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Logistics
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Test Indications:
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Bilirubin is the orange-yellow pigment derived from senescent red blood cells. It is extracted and biotransformed in the liver. Bilirubin is screened and monitored for liver disorders, such as jaundice, or liver disease, such as cirrhosis.
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Lab Testing Sections:
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Chemistry
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 hour
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Preferred: Green top (Li Heparin) tube
Alternate: Red top (plain, no gel) tube
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Draw Volume:
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0.6 mL blood
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Processed Volume:
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0.2 mL plasma/serum
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Collection:
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Routine venipuncture or heelstick
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Special Processing:
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Lab Staff: Specimen must be separated from cells within 2 hours of collection. Centrifuge specimen, remove serum/plasma aliquot into plastic sample cup. Analyze sample immediately or store at refrigerated temperatures. Protect specimen from light when stored for more than 8 hours.
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Patient Preparation:
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Remove photo therapy prior to sample collection.
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Sample Rejection:
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Unlabeled or mislabeled specimen
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Interpretive
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Reference Range:
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Age:
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Range (mg/dL):
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0 – 2 weeks:
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0.0 – 1.2 mg/dL
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>2 weeks:
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0.0 – 0.3 mg/dL
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Critical Values:
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N/A
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Limitations:
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Hemolysis of the sample falsely decreases the direct bilirubin result. Exposure to light rapidly and significantly decreases bilirubin.
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Methodology:
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Modified Jendrassik-Grof
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References:
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DBI Flex® reagent cartridge insert sheet, Siemens Healthcare Diagnostics, PN 717125.001 Issue Date 04/07/2008 Rev. B
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Clinical Significance, Dade Behring Inc., Glasgow Business Community, Mailbox 531, P.O. Box 6101, Newark, Delaware 19714
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Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition
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Biorad Liquichek Unassayed Chemistry Control Product Insert, Bio-Rad Laboratories, Irvine, CA 92618
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Biorad Liquichek Pediatric Control Product Insert, Bio-Rad Laboratories, Irvine, CA 92618
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Frequently Asked Questions (March 2008) Dade Behring, Global Products Support
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