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Lab Dept:
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Chemistry
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Test Name:
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HEMOGLOBIN, PLASMA
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General Information
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Lab Order Codes:
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PHG
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Synonyms:
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Plasma Hgb
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CPT Codes:
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83051 – Hemoglobin, plasma
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Test Includes:
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Plasma hemoglobin concentration measured in mg/dL.
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Logistics
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Test Indications:
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An increase in intravascular hemoglobin concentration is indicative of acute destruction of erythocytes (hemolysis) within the vascular system. Excessive amounts of free hemoglobin may lead to renal impairment.
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Lab Testing Sections:
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Chemistry
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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4 hours, Stat: 1 hour (7 AM – 10 PM)
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Preferred: Green top (Li Heparin) tube
Alternate: Red top tube
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Draw Volume:
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3 mL blood
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Processed Volume:
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1 mL plasma/serum
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Collection:
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Routine venipuncture, atraumatic
Note: Hemolysis due to extravascular red cell trauma invalidates the result.
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Special Processing:
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Lab Staff: Centrifuge specimen, remove plasma/serum aliquot into plastic sample cup. Ultracentrifuge all specimens prior to analysis to prevent interference from lipids. Analyze sample immediately or store at refrigerated temperatures.
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Patient Preparation:
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None
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Sample Rejection:
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Unlabeled or mislabeled specimen, traumatic collection
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Interpretive
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Reference Range:
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0 – 30 mg/dL
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Critical Values:
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N/A
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Limitations:
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Hemolysis due to extravascular red cell trauma invalidates the result.
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Methodology:
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Spectrophotometric
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References:
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Blackney GB, Dinwoodie AJ (1975) Clinical Biochem, vol 8, pp 96-103
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Mietes, Samuel (1977) Pediatric Clinical Chemistry, pp 126-127
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Hennepin County Medical Center Laboratory (1998) Hemoglobin in Serum
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Hemocue Plasma/Low Hb Photometer Operating Manual
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