Clinics and Departments

Laboratory Services

Lab Dept:

Chemistry

Test Name:

HEMOGLOBIN, PLASMA

General Information

Lab Order Codes:

PHG

Synonyms:

Plasma Hgb

CPT Codes:

83051 – Hemoglobin, plasma

Test Includes:

Plasma hemoglobin concentration measured in mg/dL.

Logistics

Test Indications:

An increase in intravascular hemoglobin concentration is indicative of acute destruction of erythocytes (hemolysis) within the vascular system. Excessive amounts of free hemoglobin may lead to renal impairment.

Lab Testing Sections:

Chemistry (all specimens analyzed on the Mpls Campus)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

4 hours, Stat: 1 hour (7 AM – 10 PM)

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Preferred: Green top (Li Heparin) tube
Alternate: Red top tube

Draw Volume:

3 mL blood

Processed Volume:

1 mL plasma/serum

Collection:

Routine venipuncture, atraumatic

Note: Hemolysis due to extravascular red cell trauma invalidates the result.

Special Processing:

Lab Staff: Centrifuge specimen, remove plasma/serum aliquot into plastic sample cup. Ultracentrifuge all specimens prior to analysis to prevent interference from lipids. Analyze sample immediately or store at refrigerated temperatures.

Patient Preparation:

None

Sample Rejection:

Unlabeled or mislabeled specimen; traumatic collection

Interpretive

Reference Range:

<30 mg/dL

ECMO Patients ONLY:

 

Normal

<30 mg/dL

Mildly elevated

30 -50 mg/dL

Moderately elevated

51 – 70 mg/dL

Critical value

>70 mg/dL

Critical Values:

N/A

Limitations:

Hemolysis due to extravascular red cell trauma invalidates the result.

Methodology:

Spectrophotometric

References:

Blackney GB, Dinwoodie AJ (1975) Clinical Biochem, vol 8, pp 96-103

Mietes, Samuel (1977) Pediatric Clinical Chemistry, pp 126-127

Hennepin County Medical Center Laboratory (1998) Hemoglobin in Serum

Hemocue Plasma/Low Hb Photometer Operating Manual



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