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Lab Dept:
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Coagulation
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Test Name:
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PLATELET FUNCTION ASSAY
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General Information
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Lab Order Codes:
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PFAS
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Synonyms:
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PFA; Platelet Function Screen
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CPT Codes:
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85576 x 2 – Platelet; aggregation (in vitro), each agent
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Test Includes:
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Collagen/Epinephrine & Collagen/ADP
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Logistics
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Test Indications:
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The test is useful in detecting platelet dysfunction which may be acquired (uremia), inherited (von Willebrand disease) or induced by inhibiting agents (aspirin).
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Lab Testing Sections:
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Coagulation
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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4 hours
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Light Blue top (Buffered Na Citrate 3.2%) tube
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Draw Volume:
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2.7 mL blood
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Processed Volume:
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Minimum: 2.7 mL whole blood
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Collection:
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● A clean venipuncture is essential.
● Patient’s with a hematocrit level >55% must have a special tube made to adjust for the hematocrit; contact laboratory for special tube.
● Mix thoroughly by gentle inversion. Deliver immediately to the laboratory at room temperature via courier or pneumatic tube. Do Not draw through a heparinized line. Do Not Refrigerate.
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Special Processing:
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Lab Staff: Do Not centrifuge.
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Patient Preparation:
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None
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Sample Rejection:
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Improper tube; clotted specimens; underfilled tube; hemolyzed specimen; patient’s hematocrit <30%; centrifuged samples; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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AGE:
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RANGE:
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<31 days
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COL/EPI 64 – 98 seconds
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COL/ADP 50 – 62 seconds
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>31 days
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COL/EPI 91 – 199 seconds
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COL/ADP 65 – 121 seconds
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Critical Values:
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N/A
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Limitations:
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Testing is invalid in patients with an HCT <30%, elevated ESR or platelet count <50,000.
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Methodology:
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Simulated in vitro platelet adhesion and aggregation
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Contraindications:
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Patient receiving anti-platelet drugs.
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References:
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Rand ML, et al (1998) Use of PFA-100 in the Assessment of Primary, Platelet-Related Hemostasis in the Pediatric Setting, Seminars in Thrombosis and Hemostasis 24:6
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Updates:
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11/5/2007: Changed required volume from 5.4 to 2.7 mL. Removed statement regarding prohibiting pneumatic tube transport.
3/3/2008: Added statement to leave at room temp and transport to lab via courier or pneumatic tube.
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