Clinics and Departments

Laboratory Services

Lab Dept:

Coagulation

Test Name:

PLATELET FUNCTION ASSAY

General Information

Lab Order Codes:

PFAS

Synonyms:

PFA; Platelet Function Screen

CPT Codes:

85576 x 2 – Platelet; aggregation (in vitro), each agent

Test Includes:

Collagen/Epinephrine & Collagen/ADP

Logistics

Test Indications:

The test is useful in detecting platelet dysfunction which may be acquired (uremia), inherited (von Willebrand disease) or induced by inhibiting agents (aspirin).

Lab Testing Sections:

Coagulation

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

4 hours

Special Instructions:

N/A

Specimen

Specimen Type:

Whole blood

Container:

Light Blue top (Buffered Na Citrate 3.2%) tube

Draw Volume:

2.7 mL blood

Processed Volume:

Minimum: 2.7 mL whole blood

Collection:

● A clean venipuncture is essential.
● Patient’s with a hematocrit level >55% must have a special tube made to adjust for the hematocrit; contact laboratory for special tube.
● Mix thoroughly by gentle inversion. Deliver immediately to the laboratory at room temperature via courier or pneumatic tube. Do Not draw through a heparinized line. Do Not Refrigerate.

Special Processing:

Lab Staff: Do Not centrifuge.

Patient Preparation:

None

Sample Rejection:

Improper tube; clotted specimens; underfilled tube; hemolyzed specimen; patient’s hematocrit <30%; centrifuged samples; mislabeled or unlabeled specimens

Interpretive

Reference Range:

AGE:

RANGE:

<31 days

COL/EPI 64 – 98 seconds

 

COL/ADP 50 – 62 seconds

   

>31 days

COL/EPI 91 – 199 seconds

 

COL/ADP 65 – 121 seconds

Critical Values:

N/A

Limitations:

Testing is invalid in patients with an HCT <30%, elevated ESR or platelet count <50,000.

Methodology:

Simulated in vitro platelet adhesion and aggregation

Contraindications:

Patient receiving anti-platelet drugs.

References:

Rand ML, et al (1998) Use of PFA-100 in the Assessment of Primary, Platelet-Related Hemostasis in the Pediatric Setting, Seminars in Thrombosis and Hemostasis 24:6

Updates:

11/5/2007: Changed required volume from 5.4 to 2.7 mL. Removed statement regarding prohibiting pneumatic tube transport.
3/3/2008: Added statement to leave at room temp and transport to lab via courier or pneumatic tube.



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services