Clinics and Departments

Laboratory Services

Lab Dept:

Coagulation

Test Name:

FACTOR V LEIDEN

General Information

Lab Order Codes:

F5L

Synonyms:

Factor V Leiden (R506Q) Mutation Test, Blood; Factor 5 Mutation Test; Leiden Mutation Detection

CPT Codes:

81241 – Coagulation factor V gene analysis, Leiden variant

Test Includes:

Factor V mutation amplification; factor V mutation digestion; factor V mutation probes; factor V mutation analysis; factor V mutation separation

Logistics

Test Indications:

Direct mutation analysis should be reserved for patients with clinically suspected thrombophilia and: APC-resistance proven or suspected by low APC-resistance ratio, Family history of the FV Leiden mutation.

Lab Testing Section:

Coagulation - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 81419/F5DNA)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

3 - 5 days

Special Instructions:

Deliver immediately to the laboratory. Please note special tube requirement. See Container

Specimen

Specimen Type:

Whole blood

Container:

Yellow top (ACD, Solution A) tube

Note: Special tube is available from the laboratory.

Draw Volume:

3 mL (Minimum: 1 mL) blood

Processed Volume:

Same as Draw Volume

Collection:

A clean venipuncture is essential. Mix thoroughly by gentle inversion.

Special Processing:

Lab Staff: Do Not centrifuge. Specimen should remain in original collection container. Store blood at room temperature. Ship at ambient temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Specimen more than 7 days old; mislabeled or unlabeled specimens

Interpretive

Reference Range:

Negative

Limitations:

This direct mutation analysis will not detect individuals with APC-resistance caused by mechanisms other than the Factor V Leiden.

While genetic disorders causing antithrombin , protein C, protein S deficiency, or hyperhomocysteinemia are independently associated with venous thromboembolism; interaction of these genetic disorders with the Factor V Leiden allele markedly compounds the risk for venous thromboembolism.

Environmental (clinical) risk factors also interact to produce venous thromboembolism.

Special coagulation clinic/laboratory and/or medical genetics consultations are available and may be especially helpful in complex cases or in situations where the diagnosis is atypical or uncertain.

Methodology:

Direct Mutation Analysis

References:

Mayo Medical Laboratories July 2013

Updates:

3/8/2004: Test moved from Fairview Diagnostic Laboratories to Mayo Medical Laboratories.
1/19/2006: CPT 2006 updates



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