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Lab Dept:
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Coagulation
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Test Name:
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FACTOR V LEIDEN
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General Information
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Lab Order Codes:
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F5L
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Synonyms:
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Factor V Leiden (R506Q) Mutation Test, Blood; Factor 5 Mutation Test; Leiden Mutation Detection
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CPT Codes:
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83891 – Isolation or extraction of highly purified nucleic acid
83892 – Molecular diagnostics; enzymatic digestion
83896 x5 – Nucleic acid probe, each
83903 – Mutation scanning by physical properties, per segment
83908 x2 – Signal amplification of patient nucleic, each sequence
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Test Includes:
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Factor V mutation amplification; factor V mutation digestion; factor V mutation probes; factor V mutation analysis; factor V mutation separation
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Logistics
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Test Indications:
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Direct mutation analysis should be reserved for patients with clinically suspected thrombophilia and: APC-resistance proven or suspected by low APC-resistance ratio, Family history of the FV Leiden mutation.
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Lab Testing Section:
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Coagulation - Sendouts
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Referred to:
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Mayo Medical Laboratories (MML Test# 81419)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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3 - 5 days
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Special Instructions:
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Deliver immediately to the laboratory. Please note special tube requirement. See Container
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Yellow top (ACD, Solution A) tube
Note: Special tube is available from the laboratory.
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Draw Volume:
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5 mL (Minimum: 2 mL) blood
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Processed Volume:
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Same as Draw Volume
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Collection:
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A clean venipuncture is essential. Mix thoroughly by gentle inversion.
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Special Processing:
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Lab Staff: Do Not centrifuge. Specimen should remain in original collection container. Store blood at room temperature. Ship at ambient temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Frozen specimen, specimen more then 5 days old, mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Negative
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Limitations:
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This direct mutation analysis will not detect individuals with APC-resistance caused by mechanisms other than the Factor V Leiden.
While genetic disorders causing antithrombin , protein C, protein S deficiency, or hyperhomocysteinemia are independently associated with venous thromboembolism; interaction of these genetic disorders with the Factor V Leiden allele markedly compounds the risk for venous thromboembolism.
Environmental (clinical) risk factors also interact to produce venous thromboembolism.
Special coagulation clinic/laboratory and/or medical genetics consultations are available and may be especially helpful in complex cases or in situations where the diagnosis is atypical or uncertain.
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Methodology:
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Direct Mutation Analysis
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References:
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Mayo Medical Laboratories May 2010
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Updates:
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3/8/2004: Test moved from Fairview Diagnostic Laboratories to Mayo Medical Laboratories.
1/19/2006: CPT 2006 updates
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