Clinics and Departments

Laboratory Services

Lab Dept:

Coagulation

Test Name:

FACTOR 13 ANTIGEN SUBUNIT A

General Information

Lab Order Codes:

F13SU

Synonyms:

Factor 13 Immunologic Assay; Factor 13A; Factor 13 Activity

CPT Codes:

85290 – Factor 13 (fibrin stabilizing)

Test Includes:

Antigen level for subunit A reported as a %.

Logistics

Test Indications:

Defective wound healing, delayed bleeding episodes and habitual abortions are symptoms of Factor 13 deficiency. The most common sites of hemorrhage are umbilical cord stump (80%), which is often spontaneous or after mild trauma.

First-line test to diagnose FXIII deficiency. Appropriate for evaluation of patients with a bleeding disorder present with normal PT, PTT, and platelet count test results. Use for monitoring FXIII therapy and for confirming abnormalities detected on FXIII qualitative assay.

Lab Testing Section:

Coagulation - Sendouts

Referred to:

Fairview University Medical Center (Fairview/Atlas Test: F13ACT) forwarded to ARUP Laboratories

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

3 – 12 days; performed Wednesdays

Special Instructions:

Patient should not be receiving heparin. If so, this should be noted on the request form. Heparin can affect certain coagulation factors or assay, preclude their performance, or cause spurious results.

Indicate when the specimen is drawn from a line or a heparin lock. Deliver immediately to the laboratory.

Specimen

Specimen Type:

Whole blood

Container:

Light Blue top (Buffered Na Citrate 3.2%) tube

Draw Volume:

2.7 mL blood in a 3 mL tube (Minimum: 1.8 mL in a 2 mL tube)
Please respect fill line on tube.

Processed Volume:

2 mL (minimum 1 mL) plasma frozen at -70. Follow directions below under Special Processing.

Collection:

Routine venipuncture. Do Not use the first 2 mL’s of blood collected.

If the patient’s hematocrit is >55%, contact laboratory to obtain a special tube.

Mix thoroughly by gentle inversion.

Special Processing:

Lab Staff: Centrifuge sample collected in blue top tube(s) for 5 minutes on the Stat Spin centrifuge, remove plasma and transfer to a 4 mL BCS sample cup(s), spin remaining plasma again for 5 minutes in the Stat Spin Centrifuge. Transfer plasma into one labeled 10x75 mL plastic tubes with 2 mL (minimum 1 mL) plasma. Freeze at -70 and send specimens on dry ice to Fairview University.

Patient Preparation:

If the patient has a coagulation abnormality, apply direct pressure to the puncture site for 10 minutes; apply pressure bandage. Instruct the patient to leave the bandage on for 12 hours.

Sample Rejection:

Improper tube; clotted samples; underfilled tubes; overfilled tubes; mislabeled or unlabeled specimens

Interpretive

Reference Range:

Age:

Reference range (%):

All ages

69 – 143%

Critical Values:

N/A

Limitations:

N/A

Methodology:

Chromogenic Assay

References:

Fairview University Diagnostic Laboratories May 2014

Updates:

5/19/2004: As of February 2004, Fairview no longer performs Subunit S as part of this test. References to Subunit S have been removed. Draw volume changed from 4.5 mL to 2.7 mL blood.
7/15/2004: Reference range changed from 66-150% to 60-130% for patients 1 year and older.
5/25/2010: Tubing patient specimens is no longer prohibited.
3/31/2011: CPT update and reference range update.
2/27/2014: Fairview now refers this test to ARUP. Updated to ARUP specification.
5/1/2014: Specimen must be processed and frozen at Children’s before sending to Fairview.



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