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Lab Dept:
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Coagulation
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Test Name:
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PROTHROMBIN FRAGMENT 1.2
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General Information
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Lab Order Codes:
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PF12
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Synonyms:
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PT 1.2
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CPT Codes:
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83520 - Immunoassay, analyte, quantitative; not otherwise specified
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Test Includes:
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Prothrombin fragment 1:2 reported in seconds.
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Logistics
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Test Indications:
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Useful for diagnosing hypercoagulable state and as a marker for DIC. Determines if a fragment of Prothrombin called F1.2 is present. This fragment is produced when the patient's coagulation system is activated.
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Lab Testing Section:
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Coagulation - Sendouts
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Referred to:
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Fairview University Medical Center (Test# PF)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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Performed once/week; results are reported within 2 – 7 days.
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Special Instructions:
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A clean venipuncture is essential.
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Light Blue top (Buffered Na Citrate 3.2 %) tube
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Draw Volume:
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2.7 mL blood in a 3 mL tube (Minimum: 1.8 mL in a 2 mL tube)
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Processed Volume:
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Specimen will be processed by reference lab facility.
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Collection:
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● Routine venipuncture or line draw.
● Do Not use the first 2 mL of blood collected.
● If the patient’s hematocrit is >55%, contact laboratory to obtain a special tube.
● Mix thoroughly by gentle inversion.
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Special Processing:
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Lab Staff: Do Not centrifuge. Send in original collection tube.
Prothrombin fragment 1.2 is unstable and must be processed and frozen within three hours of specimen collection. Ship the unprocessed whole blood specimen at room temperature. Must arrive within 3 hrs of collection.
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Patient Preparation:
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After Care: If the patient has a coagulation abnormality, apply direct pressure to the puncture site for 10 minutes; apply a pressure dressing. Instruct the patient to leave the bandage on for 12 hours.
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Sample Rejection:
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Clotted sample; Overfilled tube; Underfilled tube; specimen greater than 3 hrs from time of collection and not centrifuged, frozen and shipped on dry ice; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Age
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Range (nmol/L)
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All ages:
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0.00 - 0.22 nmol/L
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Critical Values:
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N/A
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Limitations:
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N/A
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Methodology:
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Enzyme-linked immmunosorbent assay
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References:
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Fairview University Medical Center Web Page June 2011
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Harmening DH (1997) Clinical Hematology and Fundamentals of Hemostasis
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Updates:
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6/3/2004: Draw volume changed from 4.5 mL to 2.7 mL blood.
8/7/2005: Reference range previously listed as: ≥1 year: 0.36 – 1.37 nM/L (2SD) for both males and females.
8/4/2006: Reference range update from Fairview, previously listed as Males, all ages: 0.17-0.68 nM/L, Females, all ages: 0.12-1.04 nM/L.
5/24/2010: Tubing patient specimens is no longer prohibited.
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