Clinics and Departments

Laboratory Services

Lab Dept:

Coagulation

Test Name:

HEMOPHILIA B (F9) SEQUENCING

General Information

Lab Order Codes:

HEMB

Synonyms:

Factor IX Sequencing for Hemophilia B

CPT Codes:

81405 – F9, full gene sequencing (Molecular Pathology, Level 6)

Test Includes:

Bidirectional sequencing of the entire F9 coding region and intron-exon boundaries and proximal promoter.

Logistics

Test Indications:

Severe deficiency of factor IX clotting activity is associated with spontaneous joint or deep tissue bleeding. Moderate or mild deficiency is associated with prolonged bleeding after tooth extractions, surgery, or injuries and recurrent or delayed wound healing.

Lab Testing Sections:

Coagulation - Sendouts

Referred to:

Mayo Medical Laboratories (forward to ARUP) MML Test: 90491, ARUP Test: 2001578

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

Within 21 days

Special Instructions:

Fill out Patient History form for Hemophilia A or B. Form is found on: http://www.aruplab.com/guides/ug/tests/iconpdf_48.pdf

Specimen

Specimen Type:

Whole blood

Container:

Lavender top (EDTA) tube

Alternate blood tube: Yellow (ACD Solution A or B) top tube or Pink (K2 EDTA) top tube.

Draw Volume:

3 mL (Minimum: 1 mL) blood

Processed Volume:

Same as Draw Volume

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Do Not Centrifuge. Send specimen in original collection container at refrigerated temperature. Forward promptly.

Order this test through the Mayo Link. Mayo Test 90491, ARUP test code 2001578.

Patient Preparation:

None

Sample Rejection:

Specimen collected in wrong container; mislabeled or unlabeled specimens

Interpretive

Reference Range:

Interpretive report

Critical Values:

N/A

Limitations:

Rare diagnostic errors can occur due to primer site mutations. Deep intronic mutations and gene duplications will not be detected in patients of either sex; large deletions will not be detected in females.

Methodology:

Polymerase Chain Reaction/Sequencing

References:

ARUP website November 2009
(800) 522-2787 Fax (801) 584-5249

Mayo Medical Laboratories
April 2010
(800) 533-1710

Updates:

2/12/2013: CPT udpate



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services