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Lab Dept:
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Flow and Immunology
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Test Name:
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CD34
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General Information
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Lab Order Codes:
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CD34
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Synonyms:
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Hematopoietic Progenitor Stem Cell Assay; Progenitor Cell Assay; Stem Cell Assay
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CPT Codes:
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86367 – Stem cells, total count
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Test Includes:
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Enumeration of hematopoietic progenitor stem cells using a combination of CD45/CD34 monoclonal antibodies.
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Logistics
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Test Indications:
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Flow cytometric evaluation of CD34+ cell level is necessary for measuring the hematopoietic progenitor cells in dose requirement protocols of stem cell transplantation. Quantification of CD34+ cells is also used during mobilization of hematopoietic cells and leukophoresis procedures. The total number of CD34+ cells collected during leukophoresis can be estimated.
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Lab Testing Sections:
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Flow and Immunology - Sendouts
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Referred to:
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Fairview University Diagnostic Laboratories (Test# IFC)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1-3 days, test performed Monday - Friday
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Special Instructions:
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Provide diagnosis, age, collection date and time, specimen type, WBC and leukocyte differential.
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Lavender top (EDTA) tube
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Draw Volume:
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3 mL (Minimum: 2 mL) blood
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Processed Volume:
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Specimen will be processed by reference lab facility.
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Collection:
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Aseptic collection. Mix specimen well by gentle inversion.
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Special Processing:
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Lab Staff: Do Not centrifuge. Store specimens in the refrigerator. Contact IFC lab prior to shipping. Ship specimens at ambient temperatures to arrive within 24 hours of collection.
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Patient Preparation:
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None
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Sample Rejection:
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Improper specimen collection of handling; clotted specimen, specimen received more than 48 hours after collection; mislabeled or unlabeled specimen
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Interpretive
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Reference Range:
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Results are reported as a percentage of CD45/CD34+ total nucleated cells. An interpretation is provided with each report.
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Critical Values:
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N/A
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Limitations:
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N/A
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Methodology:
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Multi-color immunofluorescence and flow cytometry
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References:
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Fairview University Diagnostic Laboratory November 2010
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Updates:
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1/18/2006: 2006 CPT updates
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