Clinics and Departments

Laboratory Services

Lab Dept:

Flow and Immunology

Test Name:

CD34

General Information

Lab Order Codes:

CD34

Synonyms:

Hematopoietic Progenitor Stem Cell Assay; Progenitor Cell Assay; Stem Cell Assay

CPT Codes:

86367 – Stem cells, total count

Test Includes:

Enumeration of hematopoietic progenitor stem cells using a combination of CD45/CD34 monoclonal antibodies.

Logistics

Test Indications:

Flow cytometric evaluation of CD34+ cell level is necessary for measuring the hematopoietic progenitor cells in dose requirement protocols of stem cell transplantation. Quantification of CD34+ cells is also used during mobilization of hematopoietic cells and leukophoresis procedures. The total number of CD34+ cells collected during leukophoresis can be estimated.

Lab Testing Sections:

Flow and Immunology - Sendouts

Referred to:

Fairview University Diagnostic Laboratories (Test: IFC)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1-3 days, test performed Monday - Friday

Special Instructions:

Provide diagnosis, age, collection date and time, specimen type, WBC and leukocyte differential.

Specimen

Specimen Type:

Whole blood

Container:

Lavender top (EDTA) tube

Draw Volume:

3 mL (Minimum: 2 mL) blood

Processed Volume:

Specimen will be processed by reference lab facility.

Collection:

Aseptic collection. Mix specimen well by gentle inversion.

Special Processing:

Lab Staff: Do Not centrifuge. Store specimens in the refrigerator. Contact IFC lab prior to shipping. Ship specimens at ambient temperatures to arrive within 24 hours of collection.

Patient Preparation:

None

Sample Rejection:

Improper specimen collection of handling; clotted specimen; specimen received more than 48 hours after collection; mislabeled or unlabeled specimen

Interpretive

Reference Range:

Results are reported as a percentage of CD45/CD34+ total nucleated cells. An interpretation is provided with each report.

Critical Values:

N/A

Limitations:

N/A

Methodology:

Multi-color immunofluorescence and flow cytometry

References:

Fairview University Diagnostic Laboratory November 2010

Updates:

1/18/2006: 2006 CPT updates



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services