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Lab Dept:
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Flow and Immunology
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Test Name:
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FLUORESCENT ANTINUCLEAR ANTIBODY SCREEN, HEP-2
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General Information
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Lab Order Codes:
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FANA
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Synonyms:
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ANA Screen; FANA Screen
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CPT Codes:
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86038 – Antinuclear antibodies (ANA)
86039 - Antinuclear antibodies (ANA); titer
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Test Includes:
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The ANA screen will be reported as Negative or Positive. The screening titer is 1:160. If positive, the pattern will also be reported and the serum will be titered.
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Logistics
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Test Indications:
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Antinuclear antibody (ANA) is a general term used to describe autoantibodies against various cell nuclear proteins. This test is useful in screening for systemic lupus erythematosus (SLE) and other connective tissue autoimmune diseases
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Lab Testing Sections:
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Immunology
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 - 4 days, batch processed twice weekly
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube, plain, no gel
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Draw Volume:
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1.5 mL blood
If ENA and/or dsDNA are also being ordered, a draw volume 1.5 mL will be adequate for all tests
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Processed Volume:
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0.5 mL serum
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Collection:
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Blood should be collected aseptically and placed in a plain red top tube or other plain sterile tube without anticoagulant and allowed to clot at room temperature.
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Special Processing:
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Lab Staff: Centrifuge specimen, transfer serum to a plain polypropylene tube, and refrigerate. If testing is delayed longer than 7 days, serum should be frozen at -20ºC or colder. Serum should not be stored in a self-defrosting freezer
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Patient Preparation:
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None
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Sample Rejection:
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Sera exhibiting a high degree of hemolysis, icterus, or microbial growth will not be used because these conditions may cause aberrant results
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Interpretive
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Reference Range:
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Negative
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Critical Values:
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None
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Limitations:
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Diagnosis cannot be made on the basis of antinuclear antibody detection alone. The physician must interpret ANA results in conjunction with the patient’s history and symptoms, the physical findings, and other diagnostic procedures.
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Methodology:
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Indirect Fluorescent Antibody (IFA) using HEp-2000® substrate
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Contraindications:
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Patients with heterophile antibodies may give equivocal results.
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References:
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Immuno Concepts® HEp-2000® Fluorescent ANA-Ro Test System Package Insert, Rev. 3 1999
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