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Lab Dept:
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Hematology
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Test Name:
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EOSINOPHILS, TOTAL COUNT
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General Information
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Lab Order Codes:
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EOCT
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Synonyms:
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Total Eos; Eo Count; Absolute Number of Eosinophils
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CPT Codes:
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85021 – Hemogram, automated
85008 – Manual blood smear examination with differential parameters
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Test Includes:
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N/A
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Logistics
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Test Indications:
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Useful in determining presence of eosinopenia or eosinophilia in the peripheral blood which can be present in a variety of conditions: adrenal response to ACTH, allergic reactions, parasitic infections, skin disorders, hypereosinophilic syndromes, neoplastic diseases and other miscellaneous disease states
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Lab Testing Sections:
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Hematology
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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4 hours
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Special Instructions:
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Do Not collect specimen while lipids are being infused or 4 hours post-infusion. Specimen must arrive within 2 hours of draw.
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Lavender top tube (EDTA) or Lavender Microtainer®
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Draw Volume:
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2.0 mL blood in a short draw 5.0 mL EDTA tube or 0.5 mL in a Microtainer®
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Processed Volume:
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Same as Draw Volume
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Collection:
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Fill to mark on tube or Microtainer®. Mix well by gentle inversion.
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Special Processing:
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Lab Staff: Do Not centrifuge
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Patient Preparation:
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None
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Sample Rejection:
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The following samples will be rejected:
Improper tube, Clotted sample, Overfilled tube, Underfilled tube
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Interpretive
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Reference Range:
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50 – 250 Eos/mm3
Eosinopenia: <50 Eos/mm3
Eosinophilia: >450 Eos/mm3
A definite diurnal variation occurs with a mid-morning nadir, approximately 20% less than the 0800 level; a maximal nocturnal peak, approximately 30% greater than the 0800 level. This diurnal variation is not observed in patients with adrenal insufficiency.
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Critical Values:
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N/A
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Limitations:
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Estimates of eosinophil numbers based on differential counts and stained blood smears are subject to error because the natural stickiness or adherence of eosinophils prevents even distribution.
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Methodology:
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Automated cell counter with automated or manual differential.
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Contraindications:
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N/A
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References:
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Davidson, Henry, Todd-Sanford (1979) Clinical Diagnosis by Laboratory Methods, 15th ed
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Harmening DH (1997) Clinical Hematology and Fundamentals of Hemostasis, 3rd ed
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Oski and Nathan (1998) Hematology of Infancy and Childhood, 5th ed
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