Clinics and Departments

Laboratory Services

Lab Dept:

Hematology

Test Name:

PNH TESTING

General Information

Lab Order Codes:

PANH

Synonyms:

Paroxysmal Nocturnal Hemoglobinuria; CD59; Pi-linked Antigen; Acid Hemolysin; FLAER; Ham-Crosby Sugar-Water Test; Sucrose Fragility

CPT Codes:

88184 – Flow cytometry; 1st marker, red blood cell
88184 – Flow cytometry; 1st marker, white blood cell
88185 x6 – Flow cytometry; each additional marker, WBC
88185 – Flow cytometry; each additional marker, RBC

Test Includes:

Flow cytometry evaluation of PI-Linked antigens and when positive will include a pathologist review and interpretation when appropriate.

Logistics

Test Indications:

The assay is useful in screening or in confirming the diagnosis of PNH and in following those patients. It will detect the presence or absence of GPI-linked antigens on the surface of peripheral blood granulocytes, monocytes, erythrocytes, and/or lymphocytes. The sugar water test and the Ham’s test are not suggested for the evaluation of patients with possible PNH.

Lab Testing Sections:

Hematology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: PLINK)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 2 days, testing set up daily

Special Instructions:

Obtain special collection tube from the laboratory. See Container

Specimen

Specimen Type:

Whole blood

Container:

Yellow top (ACD solution B) tube, preferred
Alternate tube: Lavender top (EDTA) tube

Draw Volume:

6 mL (Minimum: 1 mL) blood

Processed Volume:

Same as Draw Volume.

Collection:

Routine venipuncture. Mix thoroughly by gentle inversion.

Special Processing:

Lab Staff: Do not centrifuge. Specimen should remain in original collection container. Store and ship at ambient temperature. Must arrive within 72 hours of collection. Specimen must not be frozen.

Patient Preparation:

None

Sample Rejection:

Improper specimen collection or handling; clotted specimen; frozen specimen; mislabeled or unlabeled specimens

Interpretive
 

RBC Partial Antigen Loss
(RBC Type II)

0.00 – 0.99%

RBC Complete Antigen Loss
(RBC Type III)

0.00 – 0.01%

Granulocytes

0.00 – 0.01%

Monocytes

0.00 – 0.05%

An interpretive report will be provided

Limitations:

The sugar water test and Ham’s test are no longer recommended for the evaluation of patients with possible PNH.

Methodology:

Flow Cytometric Immunophenotyping

References:

Mayo Medical Laboratories Web Page April 2013

Updates:

3/11/04: Test moved from Allina-United Hospital Flow Cytometry Laboratory to Mayo Medical Laboratories.
4/25/2005: Updated for 2005 CPT coding. Previously listed as 88184 x4.
3/16/2010: Test updated at MML to include additional antibodies, change in CPT coding. Change in ref ranges and reporting.
4/22/2013: RBC Type I no longer reported. Updated names for RBC Types II and III.



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