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Lab Dept:
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Hematology
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Test Name:
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PNH TESTING
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General Information
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Lab Order Codes:
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PANH
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Synonyms:
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Paroxysmal Nocturnal Hemoglobinuria; CD59; Pi-linked Antigen; Acid Hemolysin; FLAER; Ham-Crosby Sugar-Water Test; Sucrose Fragility
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CPT Codes:
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88184 – Flow cytometry; 1st marker, red blood cell
88184 – Flow cytometry; 1st marker, white blood cell
88185 x6 – Flow cytometry; each additional marker, WBC
88185 – Flow cytometry; each additional marker, RBC
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Test Includes:
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Flow cytometry evaluation of PI-Linked antigens and when positive will include a pathologist review and interpretation when appropriate.
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Logistics
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Test Indications:
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The assay is useful in screening or in confirming the diagnosis of PNH and in following those patients. It will detect the presence or absence of GPI-linked antigens on the surface of peripheral blood granulocytes, monocytes, erythrocytes, and/or lymphocytes. The sugar water test and the Ham’s test are not suggested for the evaluation of patients with possible PNH.
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Lab Testing Sections:
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Hematology - Sendouts
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Referred to:
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Mayo Medical Laboratories (MML Test: PLINK)
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Phone Numbers:
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MIN Lab: 612-813-6280
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STP Lab: 651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 2 days, testing set up daily
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Special Instructions:
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Obtain special collection tube from the laboratory. See Container
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Yellow top (ACD solution B) tube, preferred
Alternate tube: Lavender top (EDTA) tube
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Draw Volume:
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6 mL (Minimum: 1 mL) blood
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Processed Volume:
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Same as Draw Volume.
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Collection:
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Routine venipuncture. Mix thoroughly by gentle inversion.
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Special Processing:
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Lab Staff: Do not centrifuge. Specimen should remain in original collection container. Store and ship at ambient temperature. Must arrive within 72 hours of collection. Specimen must not be frozen.
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Patient Preparation:
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None
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Sample Rejection:
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Improper specimen collection or handling; clotted specimen; frozen specimen; mislabeled or unlabeled specimens
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Interpretive
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RBC Partial Antigen Loss
(RBC Type II)
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0.00 – 0.99%
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RBC Complete Antigen Loss
(RBC Type III)
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0.00 – 0.01%
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Granulocytes
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0.00 – 0.01%
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Monocytes
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0.00 – 0.05%
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An interpretive report will be provided
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Limitations:
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The sugar water test and Ham’s test are no longer recommended for the evaluation of patients with possible PNH.
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Methodology:
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Flow Cytometric Immunophenotyping
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References:
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Mayo Medical Laboratories Web Page April 2013
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Updates:
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3/11/04: Test moved from Allina-United Hospital Flow Cytometry Laboratory to Mayo Medical Laboratories.
4/25/2005: Updated for 2005 CPT coding. Previously listed as 88184 x4.
3/16/2010: Test updated at MML to include additional antibodies, change in CPT coding. Change in ref ranges and reporting.
4/22/2013: RBC Type I no longer reported. Updated names for RBC Types II and III.
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