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Lab Dept:
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Hematology
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Test Name:
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RETICULOCYTE COUNT
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General Information
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Lab Order Codes:
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RETB
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Synonyms:
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Retic Panel; Retic Count, Whole Blood; Retic
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CPT Codes:
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85045 – Reticulocyte count, flow cytometry
OR
85044 – Reticulocyte count, manual
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Test Includes:
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% Retics; Immature Retic Fraction (IRF) and Absolute Retics
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Logistics
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Test Indications:
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Useful for evaluating erythropoietic activity in patients with hemolytic anemia, hemorrhage, uremia, aplastic anemia, aplastic crisis of hemolytic anemia, thalassemia, pernicious anemia, sideroblastic anemia, after bone marrow transplant, and after treatment for iron deficiency anemia and megaloblastic anemia.
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Lab Testing Sections:
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Hematology
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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4 hours
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Special Instructions:
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Specimen must arrive within 1 hour of collection. Do Not collect specimen while lipids are being infused or for 4 hours post-infusion.
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Lavender top tube (EDTA) or Lavender MicrotainerŽ
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Draw Volume:
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2.0 mL blood in a short draw 5.0 mL EDTA tube or 0.5 mL in a MicrotainerŽ
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Processed Volume:
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Same as Draw Volume
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Collection:
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Fill to mark on tube or MicrotainerŽ. Mix well by gentle inversion.
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Special Processing:
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Lab Staff: Do Not Centrifuge.
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Patient Preparation:
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None
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Sample Rejection:
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The following specimens will be rejected:
Improper tube, Clotted sample, Underfilled tube, Overfilled tube
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Interpretive
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Reference Range:
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Instrument Count:
0 - 3 days: 4.3 - 8.3%
4 days - 6 months: 1.3 - 2.7%
>6 months: 0.7 - 2.8%
Manual Miller Disc:
0 - 3 days: 2.0 - 70%
4 days - 6 months: 0.0 - 2.0%
>6 months: 0.5 - 1.5%
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Critical Values:
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N/A
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Limitations:
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In transfused patients, reticulocytes may decrease on a dilutional basis. An automated count is routinely reported, on rare occasion interfering substances mandate a manual count.
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Methodology:
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Automated Cell Counter using Flow Cytometry.
Back up Method is Manual Count using a Miller Disc.
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Contraindications:
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Patients receiving a large number of transfusions.
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References:
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Harmening DH (1997) Clinical Hematology and Fundamentals of Hemostasis, 3rd ed
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Oski and Nathan (1998) Hematology of Infancy and Childhood, 5th ed
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