Clinics and Departments

Laboratory Services

Lab Dept:

Hematology

Test Name:

SICKLE SCREEN

General Information

Lab Order Codes:

SIK

Synonyms:

Hemoglobin S Screen, Blood; Hemoglobin Solubility; Sickle Screen; Sickledex

CPT Codes:

85660 – Sickling of RBC, reduction

Test Includes:

Sickle screen reported as positive or negative.

Logistics

Test Indications:

Useful to screen for sickling hemoglobins.

Lab Testing Sections:

Transfusion Service

Phone Numbers:

612-813-6824

Test Availability:

Daily, 24 hours

Turnaround Time:

1 day

Special Instructions:

Refrigerate specimen. Should arrive within 5 days of collection.
Send in original tube

Specimen

Specimen Type:

Whole blood

Container:

Lavender top tube (EDTA) or Lavender MicrotainerŽ

Draw Volume:

2 mL in a short draw 5 mL EDTA tube or 0.5 mL in a MicrotainerŽ

Processed Volume:

Same as Draw Volume

Collection:

Fill to mark on tube or MicrotainerŽ. Mix well by gentle inversion.

Special Processing:

Lab Staff: Do Not centrifuge. Refrigerate specimen after collection.

Patient Preparation:

None

Sample Rejection:

Improper tube; clotted sample; underfilled tube; overfilled tube; mislabeled or unlabeled tube

Interpretive

Reference Range:

Negative

Critical Values:

N/A

Limitations:

Several other hemoglobins (e.g., Hemoglobin C Harlem, Hemoglobin C Georgetown, Hemoglobin C Ziguinchor) yield positive results. Positive reactions with dithionite solubility should be confirmed with hemoglobin electrophoresis.

Methodology:

Dithionite solubility

References:

Harmening DH (1997) Clinical Hematology and Fundamentals of Hemostasis, 3rd ed

Oski and Nathan (1998) Hematology of Infancy and Childhood, 5th ed

Updates:

3/4/2013: Phone reference updated.
8/5/2014: Updated specimen stability/transport under Special Instructions.



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