Lab Dept:

Hematology

Test Name:

SICKLE SCREEN

General Information

Lab Order Codes:

SIK

Synonyms:

Hemoglobin S Screen, Blood; Hemoglobin Solubility; Sickle Screen; Sickledex

CPT Codes:

85660 – Sickling of RBC, reduction

Test Includes:

N/A

Logistics

Test Indications:

Useful to screen for sickling hemoglobins.

Lab Testing Sections:

Hematology

Phone Numbers:

Minneapolis:

Saint Paul:

612-813-6280

651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 day

Special Instructions:

Specimen must arrive within 1 hour of collection. Send in original tube

Specimen

Specimen Type:

Whole blood

Container:

Lavender top tube (EDTA) or Lavender Microtainer®

Draw Volume:

2.0 mL in a short draw 5.0 mL EDTA tube or 0.5 mL in a Microtainer®

Processed Volume:

Same as Draw Volume

Collection:

Fill to mark on tube or Microtainer®. Mix well by gentle inversion.

Special Processing:

Lab Staff: Do Not centrifuge. Refrigerate specimen after collection.

Patient Preparation:

None

Sample Rejection:

The following specimens will be rejected:

Improper tube, Clotted sample, Underfilled tube, Overfilled tube

Interpretive

Reference Range:

Negative

Critical Values:

N/A

Limitations:

Several other hemoglobins (e.g., Hemoglobin C Harlem, Hemoglobin C Georgetown, Hemoglobin C Ziguinchor) yield positive results. Positive reactions with dithionite solubility should be confirmed with hemoglobin electrophoresis.

Methodology:

Dithionite solubility

Contraindications:

N/A

References:

Harmening DH (1997) Clinical Hematology and Fundamentals of Hemostasis, 3^rd ed

Oski and Nathan (1998) Hematology of Infancy and Childhood, 5^th ed