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Lab Dept:
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Hematology
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Test Name:
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SICKLE SCREEN
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General Information
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Lab Order Codes:
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SIK
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Synonyms:
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Hemoglobin S Screen, Blood; Hemoglobin Solubility; Sickle Screen; Sickledex
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CPT Codes:
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85660 – Sickling of RBC, reduction
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Test Includes:
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N/A
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Logistics
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Test Indications:
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Useful to screen for sickling hemoglobins.
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Lab Testing Sections:
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Transfusion Service
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6824
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651-220-6558
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 day
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Special Instructions:
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Specimen must arrive within 1 hour of collection. Send in original tube
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Lavender top tube (EDTA) or Lavender MicrotainerŽ
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Draw Volume:
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2 mL in a short draw 5 mL EDTA tube or 0.5 mL in a MicrotainerŽ
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Processed Volume:
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Same as Draw Volume
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Collection:
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Fill to mark on tube or MicrotainerŽ. Mix well by gentle inversion.
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Special Processing:
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Lab Staff: Do Not centrifuge. Refrigerate specimen after collection.
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Patient Preparation:
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None
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Sample Rejection:
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Improper tube; clotted sample; underfilled tube; overfilled tube; mislabeled or unlabeled tube
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Interpretive
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Reference Range:
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Negative
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Critical Values:
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N/A
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Limitations:
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Several other hemoglobins (e.g., Hemoglobin C Harlem, Hemoglobin C Georgetown, Hemoglobin C Ziguinchor) yield positive results. Positive reactions with dithionite solubility should be confirmed with hemoglobin electrophoresis.
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Methodology:
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Dithionite solubility
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References:
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Harmening DH (1997) Clinical Hematology and Fundamentals of Hemostasis, 3rd ed
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Oski and Nathan (1998) Hematology of Infancy and Childhood, 5th ed
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