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Lab Dept:
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Hematology
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Test Name:
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HEMOGLOBIN F BY FLOW CYTOMETRY
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General Information
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Lab Order Codes:
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HGBFF
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Synonyms:
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Hgb F by Flow Cytometry; Hemoglobin F, Red Cell Distribution, Blood
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CPT Codes:
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88184 – Flow cytometry, cell surface, cytoplasmic, or nuclear marker, technical component only; first marker
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Test Includes:
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The presence of hemoglobin F reported as heterocellular or homocellular.
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Logistics
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Test Indications:
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Useful in distinguishing hereditary persistence of fetal hemoglobin from other conditions with increased amounts of fetal hemoglobin.
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Lab Testing Sections:
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Hematology - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 8270)
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Phone Numbers:
Minneapolis:
Saint Paul:
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812-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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2 – 5 days, test set up Monday - Saturday
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Special Instructions:
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A current Hemoglobin F% must be submitted with an order for this test. If a current Hemoglobin F value is not available, Hemoglobin F, Blood should be ordered first or in conjunction with this test. This test is not to be ordered for fetal-maternal bleed.
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Lavender top (EDTA) tube
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Draw Volume:
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3.0 mL (Minimum: 0.5 mL) blood
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Processed Volume:
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Same as Draw Volume
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Collection:
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Routine venipuncture A Hemoglobin F% for the patient should be submitted with an order for this test. If a current Hemoglobin F value is not available, Hemoglobin F, Blood should be ordered first or in conjunction with this test. This test is not to be ordered for fetal-maternal bleed.
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Special Processing:
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Lab Staff: Do Not Centrifuge. Send specimen in original collection container. Store and ship at refrigerated temperatures. Do Not freeze. Indicate the % of Hemoglobin F patient age and sex. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Specimens other than whole blood, frozen specimens, anticoagulants other than EDTA or heparin, hemolyzed specimens, mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Reported as heterocellular or hepatocellular
Note: In the common form of the genetic trait, hereditary persistence of fetal hemoglobin (HPFH), all of the erythrocytes contain hemoglobin F.
More than 75% of the hemoglobin of the newborn is hemoglobin F, it diminishes over a period of several months to adult levels, becoming <2% by 1 year of age.
Hemoglobin F may constitute 90% of the total hemoglobin in patients with beta-thalassemia major.
Hemoglobin F is often moderately elevated in sickle cell disease, aplastic anemia, acute leukemia, myelo-proliferative disorders, hereditary spherocytosis, and alpha-thalassemia minor. It is commonly increased in all hemoglobinopathies associated with hemolysis. Hemoglobin F increases to as high as 10% during normal pregnancy.
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Critical Values:
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N/A
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Limitations:
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Indication for test must be stated.
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Methodology:
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Flow cytometry
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Contraindications:
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N/A
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References:
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Mayo Medical Laboratories Web Page February 2007
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Updates:
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2/28/2007: CPT previously listed as 88180. Update for CPT 2007.
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