Clinics and Departments

Laboratory Services

Lab Dept:

Hematology

Test Name:

HEMOGLOBIN F BY FLOW CYTOMETRY

General Information

Lab Order Codes:

HGBFF

Synonyms:

Hgb F by Flow Cytometry; Hemoglobin F, Red Cell Distribution, Blood

CPT Codes:

88184 – Flow cytometry, cell surface, cytoplasmic, or nuclear marker, technical component only; first marker

Test Includes:

The presence of hemoglobin F reported as heterocellular or homocellular.

Logistics

Test Indications:

Useful in distinguishing hereditary persistence of fetal hemoglobin from other conditions with increased amounts of fetal hemoglobin.

Lab Testing Sections:

Hematology - Sendouts

Referred to:

Mayo Medical Laboratories (Test:HPFH/8270)

Phone Numbers:

MIN Lab: 812-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

2 – 5 days, test set up Monday - Saturday

Special Instructions:

A current Hemoglobin F% must be submitted with an order for this test. If a current Hemoglobin F value is not available, Hemoglobin F, Blood should be ordered first or in conjunction with this test. This test is not to be ordered for fetal-maternal bleed.

Specimen

Specimen Type:

Whole blood

Container:

Lavender top (EDTA) tube

Draw Volume:

3 mL (Minimum: 0.5 mL) blood

Processed Volume:

Same as Draw Volume

Collection:

Routine venipuncture A Hemoglobin F% for the patient should be submitted with an order for this test. If a current Hemoglobin F value is not available, Hemoglobin F, Blood should be ordered first or in conjunction with this test. This test is not to be ordered for fetal-maternal bleed.

Special Processing:

Lab Staff: Do Not Centrifuge. Send specimen in original collection container. Store and ship at refrigerated temperatures. Do Not freeze. Indicate the % of Hemoglobin F patient age and sex. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Specimens other than whole blood, frozen specimens, anticoagulants other than EDTA or heparin, hemolyzed specimens, mislabeled or unlabeled specimens

Interpretive

Reference Range:

Reported as heterocellular or hepatocellular

Note: In the common form of the genetic trait, hereditary persistence of fetal hemoglobin (HPFH), all of the erythrocytes contain hemoglobin F.

More than 75% of the hemoglobin of the newborn is hemoglobin F, it diminishes over a period of several months to adult levels, becoming <2% by 1 year of age.

Hemoglobin F may constitute 90% of the total hemoglobin in patients with beta-thalassemia major.

Hemoglobin F is often moderately elevated in sickle cell disease, aplastic anemia, acute leukemia, myelo-proliferative disorders, hereditary spherocytosis, and alpha-thalassemia minor. It is commonly increased in all hemoglobinopathies associated with hemolysis. Hemoglobin F increases to as high as 10% during normal pregnancy.

Critical Values:

N/A

Limitations:

Indication for test must be stated.

Methodology:

Flow cytometry

References:

Mayo Medical Laboratories Web Page February 2007

Updates:

2/28/2007: CPT previously listed as 88180. Update for CPT 2007.



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