Clinics and Departments

Laboratory Services

Lab Dept:

Hematology

Test Name:

HEMOGLOBIN F, BLOOD

General Information

Lab Order Codes:

HBF

Synonyms:

Hemoglobin F; Fetal Hemoglobin; APT test; Hemoglobin F, Alkalai Resistant

CPT Codes:

83021 – Hemoglobin fractionation and quantitation; chromatography

Test Includes:

The percent presence of hemoglobin F.

Logistics

Test Indications:

Useful in quantitating the percent of fetal hemoglobin present to assist in the diagnosis of disorders with elevated levels of fetal hemoglobin.

Lab Testing Sections:

Hematology – Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: HBF)

Phone Numbers:

MIN Lab: 812-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 3 days, test set up Monday - Saturday

Special Instructions:

N/A

Specimen

Specimen Type:

Whole blood

Container:

Lavender top (EDTA) tube
Alternate: Yellow top (ACD, solution B) or Green top (Na Heparin) tubes

Draw Volume:

3 mL (Minimum: 1 mL) blood

Processed Volume:

Same as Draw Volume

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Do Not Centrifuge. Send specimen in original collection container. Store and ship at refrigerated temperatures. Do Not freeze. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Specimens other than whole blood; frozen specimens; anticoagulants other than EDTA or heparin; hemolyzed specimens; mislabeled specimens

Interpretive

Reference Range:

Age:

Range (%):

1 – 30 days

22.8 – 92.0%

1 – 2 months

7.6 – 89.8%

3 –5 months

1.6 – 42.2%

6 – 8 months

0.0 – 16.7%

9 – 12 months

0.0 – 10.5%

13 – 17 months

0.0 – 7.9%

18 – 23 months

0.0 – 6.3%

≥24 months

0.0 – 0.9%

Critical Values:

N/A

Limitations:

Hemoglobin F is elevated in newborns, reaching adult levels by 12 months. It is also commonly increased to as much as 5-10% in normal pregnancy.

Methodology:

Ion-Exchange/HPLC (high-performance liquid chromatography)

References:

Mayo Medical Laboratories Web Page August 2015

Updates:

8/19/15: Updated reference ranges



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