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Lab Dept:
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Hematology
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Test Name:
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HEMOGLOBIN S AND F MONITORING
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General Information
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Lab Order Codes:
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HBSF
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Synonyms:
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Hbg S and F Quantitation for Therapeutic Monitoring
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CPT Codes:
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83021 – Hemoglobin fractionation and quantitation; chromatography
85660 – Sickling of red blood cells, reduction (if appropriate)
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Testing includes:
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Hemoglobin S and F measurement by Cation Exchange/High-Performance Liquid Chromatography (HPLC). If hemoglobin S is detected, hemoglobin S screen will be performed when appropriate at an additional charge.
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Logistics
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Test Indications:
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Monitoring patients with sickling disorders who have received hydroxyurea or transfusion therapy. This test is not intended for diagnostic purposes; it is assumed the patient’s diagnosis is established. If the patient has never been studied, hemoglobin electrophoresis is necessary.
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Lab Testing Sections:
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Hematology - Sendouts
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Referred to:
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Mayo Medical Laboratories (MML Test# 60205)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 2 days, performed Monday - Saturday
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Special Instructions:
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This test is not intended for diagnostic purposes. See Test Indications.
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Lavender (EDTA) top tube
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Draw Volume:
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6 mL (Minimum: 1 mL) blood
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Processed Volume:
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Same as Draw Volume.
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Do Not centrifuge. Specimen should remain in original collection container. Do not freeze. Send refrigerated.
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Patient Preparation:
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None
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Sample Rejection:
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Testing cannot be performed on clotted samples; frozen specimens; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Hgb Fraction
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Age
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Range
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Hgb F
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1 – 30 days:
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22.8 – 92.0%
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1 – 2 months:
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7.6 – 89.8%
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3 – 5 months:
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1.6 – 42.2%
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6 – 8 months:
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9 – 12 months:
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13 – 17 months:
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18 – 23 months:
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>or = 24 months:
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Hgb S
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All ages:
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0.0%
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Hgb S, Screen
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All ages:
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Negative
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Critical Values:
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N/A
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Limitations:
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This test is not intended for diagnostic purposes. See Test Indications.
This assay will not discriminate hemoglobin S (Hb S) trait from homozygous sickle cell disease (S/S), nor any of the following combinations: S/C, S/D, S/G, S/E, S/beta-thalassemia, S/O-Arab, and C-Georgetown trait. The presence of HbS will be confirmed, but any additional Hb variants will not be definitively identified.
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Methodology:
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Hemoglobin F/S: Cation Exchange/HPLC
Hemoglobin Electrophoresis: Capillary Electrophoresis
Hemoglogin S: Hemoglobin S Solubility
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References:
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Mayo Medical Laboratories Lab Web Page March 2011
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