Clinics and Departments

Laboratory Services

Lab Dept:

Microbiology/Virology

Test Name:

BONE MARROW CULTURE, FUNGUS

General Information

Lab Order Codes:

BMCF

Synonyms:

Culture, Bone Marrow for Fungus; Fungus Culture, Bone Marrow

CPT Codes:

87102 – Culture, fungi isolation, with presumptive identification of isolates; skin, hair or nail, other source

The following testing may be added if appropriate based on findings for organism identification (multiple additions are possible if more than one organism is identified) and to aid in patient treatment management.

87077 – Aerobic isolate, additional methods required for definitive identification, each isolate (if appropriate)
87106 – Culture, fungi, definitive identification, each organism, yeast (if appropriate)
87107 – Culture, mold, definitive identification, each organism, mold (if appropriate)
87184 – Susceptibility studies, disk method, per plate (if appropriate)
87186 – Susceptibility studies, microdilution or agar dilution, each multi-antimicrobial, per plate (if appropriate)

Test Includes:

Culture for yeast and filamentous fungi. The physician or patient’s nurse will be notified of all positive cultures.

Logistics

Lab Testing Sections:

Microbiology

Phone Numbers:

MIN Lab: 612-813-5866

STP Lab: 651-220-6555

Test Availability:

Daily, 24 hours

Turnaround Time:

Positives are reported when detected. Negative cultures are final after 3 weeks.

Special Instructions:

Specimen site and date/time of collection are required for processing.
● If a Mycobacterium species (AFB, TB) is suspected, request Blood Culture, AFB. Refer to Bone Marrow Culture for routine bacterial culture.

Specimen

Specimen Type:

Bone marrow

Container:

Wampole ISOLATOR 1.5 microbial tube (yellow top)

Volume:

1.5 mL (Minimum: 1.0 mL) bone marrow

Collection:

1. Disinfect the stopper of the ISOLATOR tube with 70% alcohol and allow to dry.
2.
Prepare puncture site as for surgical incision.
3.
Collect 1.5 mL of blood and aseptically inoculate the ISOLATOR tube by puncturing the stopper using a blood transfer device or place an 18-gauge needle on the syringe. Removing the stopper will increase the risk of contamination. Do not force the blood into the ISOLATOR tube. This will cause the top to pop off.
4.
Gently invert the tube 4 - 5 times to mix contents.

Transport/Storage:

Transport to the Microbiology Laboratory immediately at room temperature. Do not refrigerate.

Sample Rejection:

Specimen with a transit time exceeding 2 hours after collection; specimen not submitted in appropriate transport container; improperly labeled specimen; insufficient volume; external contamination. If an unacceptable specimen is received, the physician or nursing station will be notified and another specimen will be requested before the specimen is discarded.

Interpretive

Reference Range:

No fungus isolated

Critical Values:

All positive results will be called to the physician or patient’s nurse.

Limitations:

Insufficient specimen volume decreases the recovery of organisms. The dilution of the SPS in the ISOLATOR will be inadequate and may inhibit growth.

Methodology:

Wampole ISOLATOR system

References:

Cook, JH, and M Pezzlo (1992). Specimen receipt and accessioning. Section 1. Aerobic bacteriology, 1.2.1-4. In HD Isenberg (ed) Clinical Microbiology Procedures Handbook. American Society for Microbiology, Washington DC

Miller, J Michael (1999) A Guide To Specimen Management in Clinical Microbiology, American Society for Microbiology, Washington DC

Miller, J Michael, and HT Holmes (1999) Specimen Collection, Transport, and Storage In PR Murray et al, (ed), Manual of Clinical Microbiology, 7th edition, American Society for Microbiology, Washington DC, pp 33-104

Updates:

3/23/2010: CPT updates



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