Clinics and Departments

Laboratory Services

Lab Dept:

Microbiology/Virology

Test Name:

OVA AND PARASITE EXAM, ASPIRATED SPECIMEN

General Information

Lab Order Codes:

OAP

Synonyms:

Duodenal aspirate for Ova and Parasites; O & P Exam, duodenal aspirate; O & P Exam, sputum; O & P Exam, urine; Schistosoma Exam, urine

CPT Codes:

87177 – Ova and parasites, direct smears, concentration and identification
88312 – Special stains; Group I for microorganisms, each

Test Includes:

Examination of aspirate for intestinal parasites by direct/concentrated microscopic exam and trichrome stain. If only Cryptosporidium or Giardia lamblia are requested. Refer to specific listing for Cryptosporidium/Giardia FA.

Logistics

Lab Testing Sections:

Microbiology

Phone Numbers:

MIN Lab: 612-813-5866

STP Lab: 651-220-6555

Test Availability:

Daily, test performed 0700 – 1500

Turnaround Time:

1 - 2 days

Special Instructions:

Specimen site and date/time of collection are required for specimen processing.
● Indicate travel history, clinical diagnosis or parasite suspected on requisition.

Warning: Aspirated specimens collected from a patient infected with parasites are highly infectious. Use extreme caution when Entamoeba histolytica, Hymenolepsis nana, and Taenia sp. are suspected.

Specimen

Specimen Type:

Aspirate of cyst or abscess, duodenal aspirate, mid-day urine, sigmoid aspirate, or sputum

Container:

Sterile, leakproof container

Volume:

3 - 4 mL of aspirate or entire mid-day urine collection with last voided portion containing blood and mucus.

Collection:

Duodenal Aspirate:

1. Specimen is obtained by use of a gastroduodenal tube or a fiberoptic endoscopy study, either by direct aspiration or into a trap.
2.
Place aspirate into a sterile leakproof container.
3. Transport directly to the laboratory (≤15 minutes) since specimens must be examined within 1 hour of collection.

Sigmoidoscopy:

1. Perform flexible or rigid sigmoidoscopy.
2.
Aspirate liquid from the inflamed bowel with a pipette passed through the sigmoidoscope.
3.
Place aspirate into a leakproof container.
4.
Transport directly to the laboratory (≤15 min) since specimens must be examined within 1 hour of collection.

Sputum (Expectorate):

1. Collect early morning specimen under the direct supervision of a nurse or a physician.
2.
Have patient rinse or gargle with water to remove superficial flora.
3.
Instruct patient to cough deeply to produce a lower respiratory specimen.
4.
Exam specimen to make sure it contains thick mucus. Do not submit saliva.
5.
Transport directly to the laboratory (≤15 min) since specimens must be examined within 1 hour of collection.

Urine:

1. Collect mid-day urine specimen in a sterile container. Peak egg excretion occurs between noon and 3 p.m.
2.
For patients with hematuria, eggs are associated with the terminal (last voided) portion of the specimen containing blood and mucus.
3.
Transport directly to the laboratory (≤15 min) since specimens must be examined within 1 hour of collection.

Transport/Storage:

Transport to the Microbiology Laboratory immediately at room temperature. Do not refrigerate specimen. Refrigeration inhibits the motility of certain parasites.

Sample Rejection:

Specimen with a transit time exceeding 1 hour after collection; specimen not submitted in appropriate transport container; improperly labeled specimen; insufficient volume; external contamination. If an unacceptable specimen is received, the physician or nursing station will be notified and another specimen will be requested before the specimen is discarded.

Interpretive

Reference Range:

No ova or parasites seen.

Limitations:

This procedure does not detect Cryptosporidium parvum. Refer to the specific listing for Cryptosporidium/Giardia FA.

Methodology:

Concentrated microscopic exam and trichrome stain

References:

Cook, JH, and M Pezzlo (1992). Specimen receipt and accessioning. Section 1. Aerobic bacteriology, 1.2.1-4. In HD Isenberg (ed) Clinical Microbiology Procedures Handbook. American Society for Microbiology, Washington DC

Miller, J Michael (1999) A Guide To Specimen Management in Clinical Microbiology, American Society for Microbiology, Washington DC

Miller, J Michael, and HT Holmes (1999) Specimen Collection, Transport, and Storage In PR Murray et al, (ed), Manual of Clinical Microbiology, 7th edition, American Society for Microbiology, Washington DC, pp 33-104



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services