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Lab Dept:
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Microbiology/Virology
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Test Name:
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OVA AND PARASITE EXAM, ASPIRATED SPECIMEN
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General Information
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Lab Order Codes:
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OAP
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Synonyms:
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Duodenal aspirate for Ova and Parasites; O & P Exam, duodenal aspirate; O & P Exam, sputum; O & P Exam, urine; Schistosoma Exam, urine
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CPT Codes:
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87177 – Ova and parasites, direct smears, concentration and identification
88312 – Special stains; Group I for microorganisms, each
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Test Includes:
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Examination of aspirate for intestinal parasites by direct/concentrated microscopic exam and trichrome stain. If only Cryptosporidium or Giardia lamblia are requested. Refer to specific listing for Cryptosporidium/Giardia FA.
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Logistics
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Lab Testing Sections:
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Microbiology
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-5866
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651-220-6555
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Test Availability:
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Daily, test performed 0700 – 1500
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Turnaround Time:
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1 - 2 days
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Special Instructions:
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● Specimen site and date/time of collection are required for specimen processing.
● Indicate travel history, clinical diagnosis or parasite suspected on requisition.
Warning: Aspirated specimens collected from a patient infected with parasites are highly infectious. Use extreme caution when Entamoeba histolytica, Hymenolepsis nana, and Taenia sp. are suspected.
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Specimen
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Specimen Type:
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Aspirate of cyst or abscess, duodenal aspirate, mid-day urine, sigmoid aspirate, or sputum
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Container:
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Sterile, leakproof container
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Volume:
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3.0 - 4.0 mL of aspirate or entire mid-day urine collection with last voided portion containing blood and mucus.
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Collection:
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Duodenal Aspirate:
1. Specimen is obtained by use of a gastroduodenal tube or a fiberoptic endoscopy study, either by direct aspiration or into a trap.
2. Place aspirate into a sterile leakproof container.
3. Transport directly to the laboratory (≤15 minutes) since specimens must be examined within 1 hour of collection.
Sigmoidoscopy:
1. Perform flexible or rigid sigmoidoscopy.
2. Aspirate liquid from the inflamed bowel with a pipette passed through the sigmoidoscope.
3. Place aspirate into a leakproof container.
4. Transport directly to the laboratory (≤15 min) since specimens must be examined within 1 hour of collection.
Sputum (Expectorate):
1. Collect early morning specimen under the direct supervision of a nurse or a physician.
2. Have patient rinse or gargle with water to remove superficial flora.
3. Instruct patient to cough deeply to produce a lower respiratory specimen.
4. Exam specimen to make sure it contains thick mucus. Do not submit saliva.
5. Transport directly to the laboratory (≤15 min) since specimens must be examined within 1 hour of collection.
Urine:
1. Collect mid-day urine specimen in a sterile container. Peak egg excretion occurs between noon and 3 p.m.
2. For patients with hematuria, eggs are associated with the terminal (last voided) portion of the specimen containing blood and mucus.
3. Transport directly to the laboratory (≤15 min) since specimens must be examined within 1 hour of collection.
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Transport/Storage:
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Transport to the Microbiology Laboratory immediately at room temperature. Do not refrigerate specimen. Refrigeration inhibits the motility of certain parasites.
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Sample Rejection:
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Specimen with a transit time exceeding 1 hour after collection; specimen not submitted in appropriate transport container; improperly labeled specimen; insufficient volume; external contamination. If an unacceptable specimen is received, the physician or nursing station will be notified and another specimen will be requested before the specimen is discarded.
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Interpretive
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Reference Range:
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No ova or parasites seen.
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Limitations:
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This procedure does not detect Cryptosporidium parvum. Refer to the specific listing for Cryptosporidium/Giardia FA.
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Methodology:
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Concentrated microscopic exam and trichrome stain
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References:
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Cook, JH, and M Pezzlo (1992). Specimen receipt and accessioning. Section 1. Aerobic bacteriology, 1.2.1-4. In HD Isenberg (ed) Clinical Microbiology Procedures Handbook. American Society for Microbiology, Washington DC
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Miller, J Michael (1999) A Guide To Specimen Management in Clinical Microbiology, American Society for Microbiology, Washington DC
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Miller, J Michael, and HT Holmes (1999) Specimen Collection, Transport, and Storage In PR Murray et al, (ed), Manual of Clinical Microbiology, 7th edition, American Society for Microbiology, Washington DC, pp 33-104
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