Swabs are not encouraged; however certain body sites, such as the ear canal, nasopharynx, throat and vagina are not readily collected by other means.
1. Collect aseptically and place in sterile container.
1. Specimen obtained by physician through the biopsy channel of the bronchoscope.
2. Transfer specimen into a luki tube.
1. Place brush into a sterile container with 1.0 mL of saline.
1. Procedure performed by ophthalmologist.
2. Contact Microbiology for media for direct inoculation.
3. Direct culture inoculation: BHI with 10% sheep blood, Chocolate agar, and SABS.
4. Deliver to lab ≤15 min at room temperature.
Pus, Exudate or Drainage:
1. Using a sterile needle and syringe, aspirate material from undrained abscesses.
2. Place in a sterile container.
1. Collect early morning specimen under the direct supervision of a nurse or a physician.
2. Have patient rinse or gargle with water to remove superficial flora.
3. Instruct patient to cough deeply to produce a lower respiratory specimen.
4. Exam specimen to make sure it contains thick mucus. Do not submit saliva.
1. Have patient rinse mouth with water after brushing gums and tongue.
2. With the aid of a nebulizer, have patients inhale ~25 mL of 3 to 10% sterile saline.
3. Collect the induced sputum in a sterile container.
4. Do not submit saliva.
1. Submit in sterile container.
2. For small samples, add a few drops of sterile saline to keep moist.
3. Do not allow tissue to dry out.
4. The portion of the biopsy specimen submitted for culture should be separated from the portion submitted for histopathology by the surgeon or pathologist.
1. Submit a catherized specimen.
2. Collect early-morning specimen in a sterile container.
3. 24 hour specimens are unacceptable.
1. With forceps, collect at least 10 - 12 affected hairs with the base of the shaft intact.
2. Place in sterile container.
1. Remove nail polish.
2. Wipe nail with 70% alcohol using gauze (not cotton).
3. Clip a generous portion of the affected area and collect scrapings of the excess keratin produced under the nail.
4. Place in sterile container.
1. Cleanse the affected area with 70% alcohol.
2. Gently scrape the surface of the skin at the active margin of the lesion. Do NOT draw blood.
3. Place scrapings between two clean glass slides.