Clinics and Departments

Laboratory Services

Lab Dept:

Microbiology/Virology

Test Name:

EBV DNA DETECTION BY PCR

General Information

Lab Order Codes:

EBPC

Synonyms:

Epstein-Barr Virus DNA Detection by Polymerase Chain Reaction, Quantitative Blood

CPT Codes:

87799 – Infectious agent detection by nucleic acid, quantification, each organism

Test Includes:

Detection of EBV using LightCycler Polymerase Chain Reaction.

Logistics

Test Indications:

Quantitative PCR for EBV-DNA in blood is intended as a prospective and diagnostic marker for the development of post-transplant lymphoproliferative disorders.

Lab Testing Sections:

Microbiology/Virology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 82987/QEBC)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 3 days

Special Instructions:

N/A

Specimen

Specimen Type:

Whole blood

Container:

Lavender top (EDTA) tube

Draw Volume:

5 mL (Minimum: 0.25 mL) blood

Processed Volume:

Same as Draw Volume

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Do Not centrifuge. Submit whole blood in original collection container. Store and ship at refrigerated temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Specimens other than EDTA whole blood, hemolysis; mislabeled or unlabeled specimens

Interpretive

Reference Range:

None detected

Increasing copy levels of EBV DNA in serial specimens may indicate positive post-transplant lymphoproliferative disorders (PTLD). Immunocompromised individuals with past-reactivated EBV infections who do not develop PTLD have low levels (< or = 40 copies/mL), whereas increasing levels from baseline and/or initially high levels (> or = 200 copies/mL) of viral DNA may be an early indicator of this condition. Positive results are quantitated in copies/mL.

Critical Values:

N/A

Limitations:

Serial determination of blood specimens from patients may be necessary to monitor increasing or decreasing levels of EBV-DNA.

Reportable range is 500 copies/mL to 100,000,000 copies/mL. Specimens with results <5,000 copies EBV DNA/mL include a disclaimer that states: “Results may not be reproducible due to low copy number.” Blood specimens of normal blood donors for EBV infection usually have low or undetectable levels of viral DNA.

Methodology:

LightCycler Polymerase Chain Reaction (PCR)

PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.

References:

Mayo Medical Laboratories Web Page October 2014

Updates:

3/4/2004: Test moved from Lab Corp (Viromed) to Mayo Medical Laboratories.
3/11/2005: Interpretation info added to reference range field.



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services