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Lab Dept:
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Microbiology/Virology
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Test Name:
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EBV DNA DETECTION BY PCR
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General Information
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Lab Order Codes:
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EBPC
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Synonyms:
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Epstein-Barr Virus DNA Detection by Polymerase Chain Reaction, Quantitative Blood
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CPT Codes:
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87799 – Infectious agent detection by nucleic acid, quantification, each organism
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Test Includes:
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Detection of EBV using LightCycler Polymerase Chain Reaction.
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Logistics
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Test Indications:
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Quantitative PCR for EBV-DNA in blood is intended as a prospective and diagnostic marker for the development of post-transplant lymphoproliferative disorders.
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Lab Testing Sections:
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Microbiology/Virology - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 82987)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 3 days, set-up Monday - Friday
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Lavender top (EDTA) tube
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Draw Volume:
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5 mL (Minimum: 0.25 mL) blood
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Processed Volume:
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Same as Draw Volume
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Do Not centrifuge. Submit whole blood in original collection container. Store and ship at refrigerated temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Specimens other than EDTA whole blood, hemolysis; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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None detected
Increasing copy levels of EBV DNA in serial specimens may indicate positive post-transplant lymphoproliferative disorders (PTLD). Immunocompromised individuals with past-reactivated EBV infections who do not develop PTLD have low levels (< or = 40 copies/mL), whereas increasing levels from baseline and/or initially high levels (> or = 200 copies/mL) of viral DNA may be an early indicator of this condition. Positive results are quantitated in copies/mL.
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Critical Values:
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N/A
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Limitations:
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Serial determination of blood specimens from patients may be necessary to monitor increasing or decreasing levels of EBV-DNA.
Reportable range is 500 copies/mL to 100,000,000 copies/mL. Specimens with results <5,000 copies EBV DNA/mL include a disclaimer that states: “Results may not be reproducible due to low copy number.” Blood specimens of normal blood donors for EBV infection usually have low or undetectable levels of viral DNA.
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Methodology:
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LightCycler Polymerase Chain Reaction (PCR)
PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.
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References:
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Mayo Medical Laboratories Web Page May 2010
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Updates:
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3/4/2004: Test moved from Lab Corp (Viromed) to Mayo Medical Laboratories.
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3/11/2005: Interpretation info added to reference range field.
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