Clinics and Departments

Laboratory Services

Lab Dept:

Microbiology/Virology

Test Name:

TRICHOMONAS WET PREP

General Information

Lab Order Codes:

WETP

Synonyms:

Trich Prep; Wet Prep for Trichomonas vaginalis

CPT Codes:

87210 – Smear, primary source with interpretation; wet mount for infectious agents

Test Includes:

Direct microscopic examination of specimen for motile trichomonads, exam for clue cells, yeast and WBC’s.

Logistics

Lab Testing Sections:

Microbiology

Phone Numbers:

MIN Lab: 612-813-5866

STP Lab: 651-220-6555

Test Availability:

Daily, 24 hours

Turnaround Time:

1 hour

Special Instructions:

Specimen site and date/time of collection are required for specimen processing.

Specimen

Specimen Type:

Vaginal or urethral discharges, prostatic fluid

Container:

Culturette Swab

Collection:

Urogenital: penile, urethral, or vaginal

1. Collect discharge material with culturette swab.

Transport/Storage:

On site collections: Transport culturette swab to the Microbiology lab as soon as possible after collection at room temperature.

Offsite collections:
For optimal results call a stat courier, send specimen at room temperature for delivery to Children’s laboratory within 1 hour of collection.

If the specimen cannot be sent immediately or is delayed, place specimen in swab transport medium which will keep organisms viable for approximately 24 hours.

Do not refrigerate. Refrigeration inhibits the motility of Trichomonas.

Sample Rejection:

Improperly labeled specimen; specimens with prolonged transit time (see Transport/Storage for requirements); specimen not submitted in appropriate transport container; insufficient volume; external contamination. If an unacceptable specimen is received, the physician or nursing station will be notified and another specimen will be requested before the specimen is discarded.

Interpretive

Reference Range:

No motile Trichomonas seen

Limitations:

False negative results occur in approximately 40% of women with trichomoniasis.

Methodology:

Wet mount

References:

Shimizu, Robyn Y (2010) Parasitology Section, Urogenital Specimens: Direct Saline Mount, section 9.6.6, in L. Garcia (ed) Clinical Microbiology Procedures Handbook, American Society of Microbiology, Washington DC

Miller, J Michael (1999) A Guide To Specimen Management in Clinical Microbiology, American Society for Microbiology, Washington DC

Miller, J Michael, and HT Holmes (1999) Specimen Collection, Transport, and Storage In PR Murray et al, (ed), Manual of Clinical Microbiology, 7th edition, American Society for Microbiology, Washington DC, pp 33-104

Updates:

3/9/2011: Sterile saline tubes and Amies transport are no longer required containers for this testing.
1/13/2014: Add offsite collection instructions.
7/9/2014: Added specimen processing for offsite collections.



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