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Lab Dept:
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Microbiology/Virology
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Test Name:
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TRICHOMONAS WET PREP
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General Information
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Lab Order Codes:
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WETP
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Synonyms:
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Trich Prep; Wet Prep for Trichomonas vaginalis
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CPT Codes:
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87210 – Smear, primary source with interpretation; wet mount for infectious agents
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Test Includes:
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Direct microscopic examination of specimen for motile trichomonads, exam for clue cells, yeast and WBC’s.
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Logistics
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Lab Testing Sections:
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Microbiology
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-5866
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651-220-6555
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 hour
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Special Instructions:
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Specimen site and date/time of collection are required for specimen processing.
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Specimen
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Specimen Type:
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Vaginal or urethral discharges, prostatic fluid
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Container:
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Culturette Swab
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Collection:
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Urogenital: penile, urethral, or vaginal
1. Collect discharge material with culturette swab.
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Transport/Storage:
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● Transport culturette swab to the Microbiology lab as soon as possible after collection at room temperature.
● Do not refrigerate. Refrigeration inhibits the motility of Trichomonas.
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Sample Rejection:
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Refrigerated Specimen. Specimen transit time exceeding 24 hours; specimen not submitted in appropriate transport container; improperly labeled specimen; insufficient volume; external contamination. If an unacceptable specimen is received, the physician or nursing station will be notified and another specimen will be requested before the specimen is discarded.
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Interpretive
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Reference Range:
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No motile Trichomonas seen
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Limitations:
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False negative results occur in approximately 40% of women with trichomoniasis.
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Methodology:
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Wet mount
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References:
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Shimizu, Robyn Y (2010) Parasitology Section, Urogenital Specimens: Direct Saline Mount, section 9.6.6, in L. Garcia (ed) Clinical Microbiology Procedures Handbook, American Society of Microbiology, Washington DC
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Miller, J Michael (1999) A Guide To Specimen Management in Clinical Microbiology, American Society for Microbiology, Washington DC
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Miller, J Michael, and HT Holmes (1999) Specimen Collection, Transport, and Storage In PR Murray et al, (ed), Manual of Clinical Microbiology, 7th edition, American Society for Microbiology, Washington DC, pp 33-104
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Updates:
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3/9/2011: Sterile saline tubes and Amies transport are no longer required containers for this testing.
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