Clinics and Departments

Laboratory Services

Lab Dept:

Microbiology/Virology

Test Name:

TRICHOMONAS WET PREP

General Information

Lab Order Codes:

WETP

Synonyms:

Trich Prep; Wet Prep for Trichomonas vaginalis

CPT Codes:

87210 – Smear, primary source with interpretation; wet mount for infectious agents

Test Includes:

Direct microscopic examination of specimen for motile trichomonads, exam for clue cells, yeast and WBC’s.

Logistics

Lab Testing Sections:

Microbiology

Phone Numbers:

MIN Lab: 612-813-5866

STP Lab: 651-220-6555

Test Availability:

Daily, 24 hours

Turnaround Time:

1 hour

Special Instructions:

Specimen site and date/time of collection are required for specimen processing.

Specimen

Specimen Type:

Vaginal or urethral discharges, prostatic fluid

Container:

Culturette Swab

Collection:

Urogenital: penile, urethral, or vaginal

1. Collect discharge material with culturette swab.

Transport/Storage:

On site collections: Transport culturette swab to the Microbiology lab as soon as possible after collection at room temperature.

Offsite collections:
Call a stat courier, send at room temperature.
Do not refrigerate. Refrigeration inhibits the motility of Trichomonas.
● Specimens must be processed within 1 hour of collection.

Sample Rejection:

Refrigerated Specimen; specimen not submitted in appropriate transport container; improperly labeled specimen; insufficient volume; external contamination. If an unacceptable specimen is received, the physician or nursing station will be notified and another specimen will be requested before the specimen is discarded.

Interpretive

Reference Range:

No motile Trichomonas seen

Limitations:

False negative results occur in approximately 40% of women with trichomoniasis.

Methodology:

Wet mount

References:

Shimizu, Robyn Y (2010) Parasitology Section, Urogenital Specimens: Direct Saline Mount, section 9.6.6, in L. Garcia (ed) Clinical Microbiology Procedures Handbook, American Society of Microbiology, Washington DC

Miller, J Michael (1999) A Guide To Specimen Management in Clinical Microbiology, American Society for Microbiology, Washington DC

Miller, J Michael, and HT Holmes (1999) Specimen Collection, Transport, and Storage In PR Murray et al, (ed), Manual of Clinical Microbiology, 7th edition, American Society for Microbiology, Washington DC, pp 33-104

Updates:

3/9/2011: Sterile saline tubes and Amies transport are no longer required containers for this testing.
1/13/2014: Add offsite collection instructions.
7/9/2014: Added specimen processing for offsite collections.



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