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Lab Dept:
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Microbiology/Virology
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Test Name:
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CMV BY PCR, BODY FLUID
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General Information
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Lab Order Codes:
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PCRC
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Synonyms:
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Cytomegalovirus (CMV) detection by Polymerase Chain Reaction (PCR), Spinal Fluid; CMV Detection by Real-Time PCR;
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CPT Codes:
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87496 - Infectious agent detection by nucleic acid (DNA or RNA); cytomegalovirus, amplified probe technique
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Test Includes:
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Detection of CMV by Real-Time PCR reported as positive or negative.
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Logistics
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Test Indications:
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Useful for early and sensitive detection of CMV infection in the spinal fluid and other body fluids for intervention in the management of CMV infections in acquired immunodeficiency syndrome and transplant patients and in other immunosuppressed patients in whom CMV viremia is suspected.
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Lab Testing Sections:
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Microbiology - Sendouts
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Referred to:
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Mayo Medical Laboratories (Test# 81240)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 – 3 days; test set up Monday - Saturday
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Special Instructions:
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Specimens grossly contaminated with blood may inhibit the PCR and produce false negative results.
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Specimen
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Specimen Type:
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CSF, Body fluid, Throat swab, Tissue, Urine
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Container:
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Body Fluid, Respiratory Specimen, CSF, Urine: Sterile container
Throat swab: Culture transport swab (Note: Calcium alginate-tipped swab, wood swab, or transports swabs containing gel are NOT acceptable)
Tissue: Sterile container containing 1-2 mL of sterile saline or multi-microbe medium (M5)
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Draw Volume:
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Body Fluid: 0.5 mL
Respiratory Specimen: 1.5 mL bronchial washing, bronchioalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate
Spinal Fluid: 0.5 mL
Throat swab: Swabbed specimen, place back into swab cylinder after collection
Tissue (brain, colon, kidney, liver, lung, etc): Tissue portion
Urine: 2 mL from a random collection
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Processed Volume:
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Same as Draw Volume
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Collection:
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Aseptic collection technique, deliver to the laboratory immediately after collection. Avoid hemolysis. Specify specific specimen source.
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Special Processing:
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Lab Staff: Send specimen refrigerated in a screw-capped sterile vial or other collection container specified above. Maintain sterility and forward promptly.
The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by CMV DNA is not likely.
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Patient Preparation:
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None
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Sample Rejection:
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Improperly labeled specimen. If an unacceptable specimen is received, the physician or nursing station will be notified and another specimen will be requested before the specimen is discarded.
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Interpretive
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Reference Range:
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Negative. If positive, reported as CMV DNA detected
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Critical Values:
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Positive
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Limitations:
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Specimens grossly contaminated with blood may inhibit the PCR and produce false negative results.
A negative result does not eliminate the possibility of CMV infection of the CNS.
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Methodology:
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Real-Time Polymerase Chain Reaction (PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)
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References:
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Mayo Medical Laboratories August 2010
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Updates:
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8/31/2010: Test expanded to include other specimen types beyond CSF.
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