1. Warm the CLOtestŪ before the endoscopy.
2. Check gel to make sure that the well is full and the color is yellow. Do not use if these conditions do not exist.
3. Peal back the label from the plastic slide so that you can see the gel. Do not remove the label.
4. Collect specimen with biopsy forceps, approximately 1-3 mm.
5. With a sterile needle, take biopsy sample from the biopsy forceps and push it into the yellow gel. Make sure that the tissue is buried.
6. Reseal the CLOtestŪ by pressing the label back on the plastic slide.
7. Write the name of the patient and collect date/time on the label.
8. Transport to the laboratory immediately.
H. pylori has been shown to cause active chronic gastritis and has been implicated as a primary etiologic factor in duodenal ulcer disease, gastric ulcer and nonulcer dyspepsia. By causing inflammation, H. pylori may weaken the mucosal defenses and allow acid and pepsin to disrupt the epithelium.
H. pylori produce large amounts of urease enzymes. Although urease primarily allows H. pylori to utilize urea as a nitrogen source, the breakdown of urea also produces high local concentrations of ammonia, which enables the organism to tolerate low pH. Tests for gastric urease are specific for H. pylori because mammalian cells do not produce urease and, except for H. pylori, the stomach is usually sterile.
Cook, J.H., and M. Pezzlo. 1992. Specimen receipt and accessioning. Section 1. Aerobic bacteriology, 1.2.1-4. In H.D. Isenberg (ed) Clinical Microbiology Procedures Handbook. American Society for Microbiology, Washington, D.C.
Miller, J. Michael, A Guide To Specimen Management in Clinical Microbiology, 1999, American Society for Microbiology, Washington, D.C.
Miller, J. Michael, and H.T. Holmes. 1999. Specimen Collection, Transport, and Storage In P.R. Murray et al., (ed.), Manual of Clinical Microbiology, 7th edition, American Society for Microbiology, Washington, D.C., pg 33-104