Clinics and Departments

Laboratory Services

Lab Dept:

Microbiology/Virology

Test Name:

EHRLICHIA/ANAPLASMA PCR, BLOOD

General Information

Lab Order Codes:

EAPB

Synonyms:

Ehrlichia chaffeensis; Ehrlichia ewingii; Ehrlichia muris-like; HME; Human Granulocytic Ehrlichiosis; Anaplasma phagocytophilum

CPT Codes:

87798 – Infectious Agent, Amplified Probe tech

Test Includes:

Anaplasma phagocytophilum PCR, Ehrlichia chaffeensis PCR, Ehrlichia Ewingii/canis PCR, Ehrlichia muris-like PCR.

Logistics

Test Indications:

Evaluating patients suspected of human granulocytic anaplasmosis or human ehrlichiosis.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test: 84319/EHRL)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 4 days, test performed Monday - Saturday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Lavender top (EDTA) tube

Draw Volume:

1 mL (Minimum: 0.3 mL) blood

Processed Volume:

Same as Draw Volume

Collection:

Routine venipuncture, invert specimen gently to mix.

Special Processing:

Lab Staff: Do Not centrifuge. Specimen should be sent in original collection container as whole blood. Store and ship at refrigerated temperatures. Forward promptly.

Patient Preparation:

None

Sample Rejection:

Unlabeled or mislabeled specimens; anticoagulants other than EDTA; gross lipemia

Interpretive

Reference Range:

Negative

Positive results indicate presence of specific DNA from Ehrlichia chaffeensis, Ehrlichia ewingii, Ehrlichia murris-like, or Anaplasma phagocytophilum and support the diagnosis of ehrlichiosis or anaplasmosis.

Negative results indicate absence of detectable DNA from any of these 4 pathogens in specimens, but it does not exclude the presence of the organism or active/recent disease.

Since DNA of Ehrlichia ewingii is indistinguishable from that of Ehrlichia canis by this rapid PCR assay, a positive result for Ehrichia ewingii/Ehrlichia canis indicates the presence of DNA from either of these 2 organisms.

Critical Values:

N/A

Limitations:

A negative result does not indicate absence of disease.

Inadequate specimen draw or improper conditions for storage and/or transport may invalidate test results.

This assay should not be used for screening asymptomatic individuals, and should only be used to test patients with signs and symptoms of ehrlichiosis/anaplasmosis.

This test may detect DNA of Ehrlichia canis (reported to cause asymptomatic infection in Venezuela only).

This PCR test does not detect DNA of Rickettsia, formerly Ehrlichia sennetsu, which has been reported to cause a rare mononucleosis-like illness in humans (in Japan and Malaysia).

Methodology:

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization.

References:

Mayo Medical Laboratories June 2013



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