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Lab Dept:
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Anatomic Pathology
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Test Name:
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MUSCLE BIOPSY
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General Information
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Synonyms:
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Muscle biopsy
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CPT Codes:
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Varies bases on specimen submitted and physician request:
88300 – Level I, Surgical pathology, gross examination only
88305 – Level IV - Surgical pathology, gross and microscopic examination (specific specimen types)
88313 x4 – Special Stains, Group II 88319-90 - Determinative histochemistry or cytochemistry to identify enzyme constituents, each
88319 x11 – Special stain including interpretation and report; Group III, for enzyme constituents
88348 – Electron microscopy, diagnostic
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Test Includes:
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A battery of histochemical stains and electron microscopy (EM) when indicated.
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Logistics
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Test Indications:
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Useful for evaluating muscle disease in terms of primary myopathy, neurogenic atrophy, etc.
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Lab Testing Section:
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Anatomic Pathology - Sendouts
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Referred to:
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Hennepin County Medical Center - Neuromuscular Lab (612)873-2175
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Phone Numbers:
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MIN Lab: 612-813-6280
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STP Lab: 651-220-6550
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Test Availability:
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Monday - Friday
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Turnaround Time:
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14 days
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Special Instructions:
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Pathology order form(s) must be filled out and submitted with specimen. Specimen must be received by 2PM on the day of the procedure at HCMC. Please notify pathology laboratory 24 hours prior to specimen collection. Obtain the biopsy from a muscle that is definitely affected, but not so severely affected that much of it is replaced by fatty or fibrous connective tissue. In addition, the involved muscle should not be one that has been traumatized by injections or by electromyographic studies. Typically, the triceps, biceps or vastus lateralis are chosen. (We find deltoid or gastrocnemius muscles less satisfactory and more subject to artifact.)
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Specimen
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Specimen Type:
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Tissue
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Container:
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For first two specimens: wrapped in saline-moistened gauze on wet ice.
For third specimen: muscle piece that is held at resting length by sutures on a piece of tongue blade immersed in EM fixative.
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Draw Volume:
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Two unfixed muscle pieces 3 - 5 mm in diameter by 1 cm in length for each specimen.
Third specimen: Muscle piece 2 mm in diameter
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Processed Volume:
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Same as Draw Volume
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Collection:
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Send 2 unfixed muscle pieces, wrapped in saline-moistened gauze on wet ice. Recommended size: 3 - 5 mm in diameter by 1 cm in length for each. Send a third muscle piece that is held at resting length by sutures on a piece of tongue blade immersed in EM fixative. Recommended size: 2 mm in diameter. If biochemical studies are anticipated, additional muscle specimens must be quickly frozen and in foil, in surgery with liquid nitrogen. The frozen tissue is stored in lab’s -70 freezer until biochemical orders/requests are received.
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Special Processing:
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See Collection
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Patient Preparation:
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Physician preference
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Sample Rejection:
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Improperly collected specimens; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Interpretive report
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References:
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Hennepin County Medical Center Laboratory Web Page http://www.hcmc.org/a_z/Neuromuscular/NeuroSpecimen.html
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Updates:
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2/12/2013: CPT update
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