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Lab Dept:
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Anatomic Pathology
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Test Name:
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GENE REARRANGEMENT, T CELL, BLOOD
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General Information
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Lab Order Codes:
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GET
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Synonyms:
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Gene-T; T-Cell Receptor Rearrangement
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CPT Codes:
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81342 – TRG@, gene rearrangement analysis, evaluation to detect abnormal clonal populations
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Test Includes:
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T-cell receptor gamma chain rearrangement by PCR. TCR beta chain by PCR per request. TCR beta, gamma or delta chains by Southern transfer per request at an additional charge.
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Logistics
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Test Indications:
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Evaluation for clonal rearrangement of T-cell receptor genes and adjunct in diagnosing lymphomas and leukemias of T-cell lineage.
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Lab Testing Section:
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Anatomic Pathology - Sendouts
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Referred to:
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Fairview University Diagnostic Laboratories
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Phone Numbers:
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MIN Lab: 612-813-6280
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STP Lab: 651-220-6550
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Test Availability:
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For best results, specimens should arrive by 2:00 PM Friday.
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Turnaround Time:
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Results are reported within 7-10 days.
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Special Instructions:
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Indicate on request form if Southern transfer technique is desired. Obtain special yellow top tubes (Solution A) from the laboratory.
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Specimen
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Specimen Type:
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Whole blood
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Container:
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Yellow top (ACD Solution A) tube
Alternate container: Lavender top (EDTA) tube
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Draw Volume:
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10 mL (Minimum: 5 mL) blood
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Processed Volume:
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Same as Draw Volume
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Collection:
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Contact Molecular Diagnostic Laboratory prior to specimen collection
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Special Processing:
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Store at room temperature. Do Not freeze. Contact laboratory prior to shipping. Ship blood at room temperature to arrive within 24-hours. Specimens collected on the weekend should be shipped to FUMC for appropriate storage.
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Patient Preparation:
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None
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Sample Rejection:
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Clotted blood; frozen specimen; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Results are reported as negative (germline or no rearrangement noted) or positive (clonal rearrangement indicating clonal cell populations present). Correlations with immunophenotypic, histopathologic, and cytogenetic data will be included with the interpretation of the molecular data when available.
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Critical Values:
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N/A
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Limitations:
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N/A
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Methodology:
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Amplification of DNA and confirmation by RFLP (restriction fragment length polymorphism) analysis using Southern transfer technique when appropriate.
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References:
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Fairview University Diagnostic Laboratory Web Page January 2012
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Updates:
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2/6/2013: CPT update
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