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Lab Dept:
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Anatomic Pathology
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Test Name:
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CILIA BIOPSY
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General Information
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Lab Order Codes:
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Orders are communicated via the Surgical Pathology request form
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Synonyms:
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Ciliary biopsy; Cilia motility; Immotile cilia; Cilia B Frequency
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CPT Codes:
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88305 – Level 4, surgical pathology, gross and microscopic exam
88329 – Pathology consult during surgery
88348 – Electron microscopy, each sampled site
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Test Includes:
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Electron microscopy (EM) of Cilia biopsy
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Logistics
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Test Indications:
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Useful for evaluating cilia for motility and ultrastructure in cases of chronic upper respiratory infection and/or suspected immotile cilia syndrome or Kartegener Syndrome.
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Lab Testing Sections:
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Anatomic Pathology – Sendouts
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Referred to:
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Mayo Medical Laboratories
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Monday - Thursday
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Turnaround Time:
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7 - 21 days
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Special Instructions:
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Obtain the biopsy from the upper airways or the carina. The mucosa over the adenoids is more likely to yield an interpretable sample of cilia; turbinate biopsies frequently show inflammation-associated loss of ciliated epithelium.
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Specimen
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Specimen Type:
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Tissue
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Container:
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Biopsy for electron microscopy: obtain container with fixative from laboratory.
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Draw Volume:
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See Collection
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Processed Volume:
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See Collection
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Collection:
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Specimen for direct observation of motility: transport fresh to lab immediately in lactate ringers. Solution can be obtained from the Histology Lab.
Specimen for electron microscopy: place in fixative immediately upon biopsy.
Trump's fixative (4% formalin: 1% glutaraldehyde) (Supply T130) is optimal. If no tissues fixed primarily in glutaraldehyde are available, shavings can be taken from the surface of tissues in formalin, where fixation was most rapidly achieved.
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Special Processing:
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N/A
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Patient Preparation:
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Physician preference
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Sample Rejection:
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Improper specimen, unlabelled or mislabeled specimen
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Interpretive
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Reference Range:
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Interpretive report (final reports will be scanned into the HIS and copies distributed to the ordering MD and surgeon)
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Critical Values:
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N/A
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Limitations:
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Artifactual loss of cilia or secondary loss of cilia due to chronic inflammatory changes, insufficient cilia numbers for definite diagnosis by electron microscopy.
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Methodology:
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Direct semi-phase-contrast microscopy, routine light microscopy, transmission electron microscopy
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References:
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Pathology Department, Children’s Hospitals and Clinics
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Mayo Medical Laboratories, February 2012
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