|
|
Lab Dept:
|
Anatomic Pathology
|
Test Name:
|
DNA MARKER, PRE BMT ENGRAFTMENT, RECIPIENT
|
General Information
|
Lab Order Codes:
|
DNAM
|
Synonyms:
|
Bone Marrow Transplant DNA Marker; DNA Polymorphism (RFLP) Molecular; Pre-Transplant Chimerism Study; DNA Marker Pre-Bone Marrow Transplant Engraftment
|
CPT Codes:
|
81265 – Comparative analysis using Short Tandem Repeat (STR) markers; patient and comparative specimen
|
Test Includes:
|
Bone Marrow Transplant DNA processing, DNA pre-BMT (Bone Marrow Transplant) polymorphism
|
Logistics
|
Test Indications:
|
Evaluation for identification of informative polymorphic markers which are used to determine engraftment status subsequent to bone marrow transplantation. An informative marker is defined as one that demonstrates unique identity for recipient and donor DNA.
|
Lab Testing Sections:
|
Anatomic Pathology – Sendouts
|
Referred to:
|
Fairview University Medical Center (Fairview Order code: MD-RCENFT)
|
Phone Numbers:
|
MIN Lab: 612-813-6280
|
STP Lab: 651-220-6550
|
Test Availability:
|
Monday – Friday (Friday specimens will be accepted until 1200 and must be received at Fairview by 1400)
|
Turnaround Time:
|
1 –2 weeks
|
Special Instructions:
|
Record the transplant recipient’s name on the request form when sending donor specimen. (Donor specimen is requested for dnenft testing.)
|
Specimen
|
Specimen Type:
|
Bone marrow or blood
|
Container:
|
Preferred: Yellow top (ACD-Solution A) tube
Alternate: Lavender top (EDTA) tube
|
Draw Volume:
|
5 mL (Minimum: 2 mL) bone marrow or blood (10 mL of blood preferred)
|
Processed Volume:
|
Same as draw volume
|
Collection:
|
Bone marrow aspiration or venipuncture
|
Special Processing:
|
Do Not centrifuge. Store bone marrow in original collection container at room temperature. Contact Fairview University prior to sending specimen.
|
Patient Preparation:
|
None
|
Sample Rejection:
|
Clotted specimen, incorrect storage temperature, unlabeled specimen or mislabeled specimen, incorrect anticoagulant
|
Interpretive
|
Reference Range:
|
Results are reported as "Found" to indicate that there is a polymorphism identified that will allow the lab to follow the DNA engraftment of the patient post-bone marrow transplant or "None" to indicate that there is not a polymorphism and the DNA engraftment of the patient cannot be followed post bone marrow transplant. The name of the donor is included to assure that the correct donor is linked to the recipient.
|
Critical Values:
|
N/A
|
Limitations:
|
N/A
|
Methodology:
|
PCR amplification of DNA and electrophoresis
|
References:
|
Fairview University Laboratory Web Page September 2008
|
Updates:
|
1/18/2006: CPT 2006 updates
3/20/2007: CPT 2007 updates
9/29/2008: CPT and price updates
2/5/2013: CPT update
|
