Lab Dept:

Anatomic Pathology

Test Name:

BCR-abl: PHILADELPHIA CHROMOSOME PCR, BLOOD

General Information

Lab Order Codes:

BRCA

Synonyms:

BCR abl Blood by PCR; Breakpoint Cluster Region (BCR) Analysis; BCR/abl t(9;22)

CPT Codes:

83891 – Isolation or extraction of highly purified nucleic acid

83898 x2 – Amplification of patient nucleic acid, single primer pair, each primer pair

83902 – Reverse transcription

83908 - Molecular diagnostics; signal amplification of patient nucleic acid, each nucleic sequence

83909 - Molecular diagnostics; separation and identification by high resolution technique

Test Includes:

PCR testing of blood for BCR. An interpretive report of the findings will be issued.

Logistics

Test Indications:

N/A

Lab Testing Sections:

Anatomic Pathology - Sendouts

Referred to:

University of Texas MD Anderson Cancer Center

Phone Numbers:

Minneapolis:

Saint Paul:

612-813-6280

651-220-6550

Test Availability:

Monday – Thursday

Turnaround Time:

15 days

Special Instructions:

Specimens must be received at MD Anderson Monday – Thursday only. No deliveries will be accepted on weekends or holidays.

Specimen

Specimen Type:

Whole blood

Container:

Lavender top (EDTA) tube

Draw Volume:

20 mL (Minimum: 10 mL) blood

Processed Volume:

Same as Draw Volume

Collection:

Routine venipuncture.

Special Processing:

Lab Staff: Do Not centrifuge. Submit specimen in original collection container. Store at room temperature and ship on a cold pack. Do Not freeze. Specimens should be labeled with patient's full name, Date of birth, patient's University of Texas ID number (if registered through UTMDA Outreach department 1-900-315-8424), date and time of collection and initials of phlebotomist. Specimens can only be shipped Monday – Thursday. Ship FedEx priority overnight.

Patient Preparation:

None

Sample Rejection:

Frozen specimen, mislabeled or unlabeled specimens

Interpretive

Reference Range:

An interpretive report will be sent.

Critical Values:

N/A

Limitations:

N/A

Methodology:

Real Time PCR with Nested and Competitive AMP as appropriate.

Contraindications:

N/A

References:

University of Texas MD Anderson Cancer Center, January 2007

Updates:

1/12/2007: CPT updates