Lab Dept:

Anatomic Pathology

Test Name:

HPV TYPING TO MAYO, TISSUE

General Information

Lab Order Codes:

HPTY

Synonyms:

Human Papilloma Virus Typing In Situ

CPT Codes:

88365 x2 – Tissue in situ hybridization, interpretation and report

Test Includes:

Positive or negative values for specific HPV DNA types in human-embedded tissue.

Logistics

Test Indications:

The detection of specific HPV DNA types: 6 and 11, and /or 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 66.

Lab Testing Sections:

Anatomic Pathology - Sendouts

Referred to:

Mayo Medical Laboratories (MML Test#: 80172)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 - 4 days

Special Instructions:

A pathology/diagnostic report and a brief history are required for processing.

Specimen

Specimen Type:

Formalin-fixed, paraffin embedded tissue block; 4 unstained glass, "positively charged" slides with formalin-fixed, paraffin-embedded tissue, or formalin-fixed tissue.

Container:

Tissue block, slides or fixed tissue

Collection:

Routine tissue collection

Special Processing:

Lab Staff: Send appropriate specimen(s) and pathology/diagnostic report and a brief history with the specimen at room temperature.

Patient Preparation:

None

Sample Rejection:

Inappropriate specimen; mislabeled or unlabeled specimen

Interpretive

Reference Range:

Results are reported as positive or negative for types 6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 66. If additional interpretation/analysis is needed, please request a surgical pathology consultation.

Interpretation: HPV types 6 and 11 are the predominant viruses associated with condyloma acuminata (genital warts). This condition usually remains benign. Other types frequently are found in CIN III (severe dysplasias and carcinoma in situ) and cervical cancer.

Critical Values:

N/A

Limitations:

This assay has been designed for use with buffered, formalin-fixed, paraffin-embedded human tissue. It has not been tested extensively with frozen tissue and is not qualified for use with other fixatives. A negative results does not exclude the presence of HPV infection. Failure to detect target DNA may be a result of such factors as improper sampling, fixation, and/or processing of target DNA at levels below the sensitivity of this assay. Some tissues with morphological changes indicative of HPV infection may not be reactive with the HPV probes currently available. Other HPV types may appear in anogenital lesions but are not detectable with the probes provided using the hybridization conditions employed in this assay. Not used for the detection of any other types than those listed under reference range above.

Methodology:

Nucleic Acid Hybridization - In Situ

References:

Mayo Medical Laboratories September 2008

Updates:

2/6/2013: CPT update