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Lab Dept:
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Serology
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Test Name:
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HEPATITIS C ANTIBODY (ANTI-HCV) REFLEX TO CONFIRMATION
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General Information
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Lab Order Codes:
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HEPC
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Synonyms:
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Anti Hepatitis C; Hepatitis C Antibody; HCVAB, Hepatitis C Viral Antibody; HCV Ab
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CPT Codes:
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86803 – Hepatitis C antibody
87522 – Hepatitis C, quantification (if appropriate)
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Test Includes:
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If Hepatitis C Virus (HCV) antibody screen by chemiluminescence immunoassay is reactive, then the presence of antibodies is confirmed by RIBA at an additional charge.
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Logistics
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Test Indications:
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Screening for past (resolved) or chronic hepatitis C with automatic reflex to antibody confirmation test.
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Lab Testing Sections:
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Serology - Sendouts
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Referred to:
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Mayo Medical Laboratories (MML Test# 15055)
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Phone Numbers:
Minneapolis:
Saint Paul:
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612-813-6280
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651-220-6550
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Test Availability:
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Daily, 24 hours
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Turnaround Time:
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1 - 2 days, test performed daily
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Special Instructions:
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N/A
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Specimen
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Specimen Type:
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Blood
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Container:
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Red top tube
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Draw Volume:
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4.5 mL (Minimum: 3 mL) blood
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Processed Volume:
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1.5 mL (Minimum: 1 mL) serum
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Collection:
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Routine venipuncture
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Special Processing:
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Lab Staff: Centrifuge specimen within 1 hour of collection, remove serum aliquot into screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.
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Patient Preparation:
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None
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Sample Rejection:
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Gross lipemia; gross hemolysis; grossly icteric; warm specimens; mislabeled or unlabeled specimens
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Interpretive
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Reference Range:
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Negative (reported as negative or positive)
Note: Positive results will be confirmed by Recombinant Immunoblot Assay (RIBA) at an additional charge.
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Critical Values:
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N/A
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Limitations:
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Serologic tests are not useful for detection or diagnosis of acute hepatitis C Virus (HCV) (< 2 months from exposure). Immunocompromised patients may not develop detectable anit-hepatitis C virus (anti-HCV) until 6 months after infection. Testing for Hepatitis C Virus RNA Detection and Quantification by Real-Time Reverse Transcription-PCR, Serum is recommended for detection of HCV infection in such patients.
Serologic test results are not useful for differentiating between past (resolved) and chronic hepatitis C. Such differentiation is best determined by detection of HCV RNA Detection and Quantification by Real-Time Reverse Transcription-PCR, Serum.
Infants born to HCV-infected mothers may have false-reactive HCV antibody test results due to transplacental passage of maternal HCV IgG antibodies. HCV antibody testing is not recommended until at least 18 months of age in these infants.
Performance characteristics have not been established for the following types of serum specimen:
● Individuals of <10 years of age
● Grossly icteric (total bilirubin level of >20 mg/dL)
● Grossly lipemic (triolein level of >3,000 mg/dL)
● Grossly hemolyzed (hemoglobin level of >500 mg/dL)
● Presence of particulate matter
● Cadaveric specimens
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Methodology:
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Chemiluminescence immunoassay (CIA)
RT-PCR for confirmation, if appropriate
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References:
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Mayo Medical Laboratories April 2011
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Updates:
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4/6/2004: Test moved from Memorial Blood Center of Minneapolis to Mayo Medical Laboratory. Test now includes an automatic reflexive confirmatory test done by RIBA when the EIA is positive.
3/9/2005: Test method previously listed as EIA. EDTA removed as an acceptable specimen anticoagulant.
11/12/2007: Name update to remove confirmation method from title.
2/25/2011: Confirmation by RIBA no longer available. Confirmation now by RT-PCR.
4/26/2011: Confirmation by RIBA once again available and will be the reflex.
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