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Lab Dept:


Test Name:


General Information

Lab Order Codes:



Human Immunodeficiency Virus Types 1 and 2 (combined) Antibodies; Anti-HIV-1,2; Anti-HIV

CPT Codes:

86703 – HIV-1 and HIV-2, single assay
86702 – HIV antibody-type 2 (if appropriate)
86689 – HIV confirmation for type 1 (if appropriate)
86689 – HIV-1 IFA (if appropriate)
86689 – HIV confirmation for type 2 (if appropriate)

Test Includes:

HIV-1,2 Antibody reported as negative or reactive. If HIV-1,2 antibody is reactive, then HIV-1,2 confirmation is performed at an additional charge. If HIV-1 Western blot is negative, then HIV-2 is performed at an additional charge. If the HIV-1 Western blot is indeterminate or uninterpretable, then both HIV-1 immunofluorescence and HIV-2 antibody screen are performed at an additional charge. If HIV-2 is reactive, then HIV-2 antibody confirmation will be performed at an additional charge.


Test Indications:

Screening and confirmation of HIV-1 and HIV-2 infection.

Lab Testing Sections:

Serology - Sendouts

Referred to:

Mayo Medical Laboratories (Test# HIVE/9333)

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 – 2 days, tests performed daily. Confirmatory testing may increase turnaround times.

Special Instructions:

This test is not offered as a screening or confirmatory test for blood donor specimens. See Patient HIV Testing Algorithm ( ) for more information.


Specimen Type:



Red top tube

Draw Volume:

3 mL (Minimum: 1.5 mL) blood

Processed Volume:

1 mL (Minimum 0.5 mL) serum/plasma


Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen within 24 hours, remove serum/plasma aliquot into screw-capped round bottom plastic vial. Store and ship at frozen temperatures. Forward promptly.

Patient Preparation:


Sample Rejection:

Specimens other than serum/plasma as indicated; lithium heparin samples are unacceptable; mislabeled or unlabeled specimens; gross hemolysis; gross lipemia; grossly icteric


Reference Range:

Negative (reported as negative or reactive)

Additional testing at an additional charge will be performed if the specimen is reactive. Refer to Test Includes:

Critical Values:



This test is not offered as a screening test for blood donor specimens.

This test is not indicated for screening or confirmatory testing of patients with reactive results obtained using HIV antibody tests.

The predictive value of a reactive or negative screening test is highly dependent on the prevalence of HIV infection in the population tested. The lower the prevalence of HIV infection, the lower the positive predictive value and higher the false-positive rate for the test.

Negative HIV-1/2 antibody screening test results should be evaluated cautiously in patients with clinical symptoms and/or a history of high-risk behavior for HIV infection. The Centers for Disease Control (CDC) recommends confirmatory testing by Western Blot on serum specimens that are reactive by the rapid HIV antibody tests; even if the initial screening results are negative.

Serologic tests (screening and confirmatory) for HIV-1/2 antibodies cannot distinguish between active neonatal HIV infection and passive transfer of maternal HIV antibodies in infants during the postnatal period (up to 18 months). Diagnosis of HIV infection in newborns and infants should be made by virologic tests such as detection of HIV RNA or HIV proviral DNA.

Performance characteristics have not been established for the following specimen characteristics: Individuals <2 months of agae; grossly hemolyzed; grossly lipemic; grossly itcteric; containing particulate matter.


HIV-1, 2 Screen and HIV-2 Aby: Chemoluminescence (EIA)
HIV-1 Aby: Indirect Immunofluorescence
HIV-1 Aby Confirmation: Western Blot
HIV-2 Aby Confirmation: Immunoassay


Mayo Medical Laboratories Web Page March 2013


4/6/2004: Test moved from Memorial Blood Center of Minneapolis to Mayo Medical Laboratories. Note: Test now reflexes to supplemental/confirmatory testing (with additional charges) when indicated by reactive findings.
11/18/2008: Method change, previously listed as Enzyme Immunoassay
3/13/2012: EDTA no longer appropriate tube type.
3/1/2013: HIV-2 confirmation now preformed by immunoassay at Mayo.

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