Clinics and Departments

Laboratory Services

Lab Dept:

Serology

Test Name:

GLIADIN, DEAMIDATED, ANTIBODIES (IgG & IgA)

General Information

Lab Order Codes:

DGP

Synonyms:

Deamidated Gliadin Antibodies, IgA and IgG, serum; Antigliadin Antibodies; Antigliadin Ab

CPT Codes:

83516 x2 – Immunoassay, analyte, qualitative; multiple step method

Test Includes:

Deamidated Gliadin Antibodies: IgG, IgA levels reported in Elia U/mL.

Logistics

Test Indications:

Evaluating patients suspected of having celiac disease, including patients with symptoms compatible with celiac disease, patients with atypical symptoms, and individuals at increased risk of celiac disease.

Monitoring compliance for a gluten-free diet.

Lab Testing Sections:

Chemistry – Minneapolis Campus only

Phone Numbers:

MIN Lab: 612-813-6280

STP Lab: 651-220-6550

Test Availability:

Daily, 24 hours

Turnaround Time:

1 - 4 days, test performed Tuesday & Friday

Special Instructions:

N/A

Specimen

Specimen Type:

Blood

Container:

Red top tube

Draw Volume:

1.5 mL (Minimum: 0.9 mL) blood

Processed Volume:

0.5 mL (Minimum: 0.3 mL) serum

Collection:

Routine venipuncture

Special Processing:

Lab Staff: Centrifuge specimen, remove serum aliquot into a screw-top plastic vial. Store at refrigerated temperatures. Freeze at or below -20˚C if not tested within 48 hours.

Patient Preparation:

None

Sample Rejection:

Mislabeled or unlabeled specimens

Interpretive

Reference Range:

Deamidated Gliadin IgA (AGP) Antibodies (all ages)

<7 Elia U/mL

Negative

7 – 10 Elia U/mL

Equivocal

>10 Elia U/mL

Positive

Deamidated Gliadin IgG (GGP) Antibodies (all ages)

<7 Elia U/mL

Negative

7 – 10 Elia U/mL

Equivocal

>10 Elia U/mL

Positive

Interpretation:
AGP >10 Elia U/mL or AGP and GGP >10 Elia U/mL and patient <7 years: Possible Celiac disease, recommend HLA, EMA or biopsy

Critical Values:

N/A

Limitations:

N/A

Methodology:

Fluoroenzyme immunoassay

References:

Phadia AB EliA Directions for Use (August 2010)

CLSI document H18-A3 (Volume 24 Number 38): Procedures for the Handling and Processing of Blood Specimens; Approved Guidelines – Third Edition

Jacobs & DeMott Laboratory Test Handbook (2001) Lexi-Comp, Inc, Hudson, OH, 5th Edition, 2001

Updates:

4/14/2004: Test moved from Specialty Laboratories to Mayo Medical Laboratories.
10/9/2007: Mayo implemented the use of an ELISA assay that detects deamidated gliadin. Note new reference ranges.
11/2/2009: Testing moved from MML to testing internally at Children’s.
5/15/2013: Move from Gliadin IgA/IgG to Deamidated Gliadin IgA/IgG. See updated reference ranges.



  Lab Test Directory
    Chemistry
    Coagulation
    Flow/Immunology
    Hematology
    Microbiology/ Virology
    Other Fluids
    Pathology
    Serology
    Transfusion Services
    Urine/Stool

  Search Lab Test Directory
    List All Documents

  Lab Staff

  E-mail Lab Services